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Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- MA1-19129 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD56 Monoclonal Antibody (MEM-188)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with an extracellular epitope on a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested. Antigen-mediated heat retrieval in citrate buffer is required for staining of paraffin-embedded sections.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MEM-188
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Differences in Peripheral Blood Lymphocytes between Brand-Name and Generic Tacrolimus Used in Stable Liver Transplant Recipients.
LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells.
Effect of Anti-IL-15 Administration on T Cell and NK Cell Homeostasis in Rhesus Macaques.
Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1.
New IDH1 mutant inhibitors for treatment of acute myeloid leukemia.
Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.
Impaired Th17 polarization of phenotypically naive CD4(+) T-cells during chronic HIV-1 infection and potential restoration with early ART.
Grb10 deletion enhances muscle cell proliferation, differentiation and GLUT4 plasma membrane translocation.
The endogenous danger signals HSP70 and MICA cooperate in the activation of cytotoxic effector functions of NK cells.
Adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells involves CD44.
A new protocol for multiple inhalation of IFN-gamma successfully treats MDR-TB: a case study.
Unique characteristics of NK cells throughout the human female reproductive tract.
Isolation of large numbers of mesenchymal stem cells from the washings of bone marrow collection bags: characterization of fresh mesenchymal stem cells.
TLRs mediate IFN-gamma production by human uterine NK cells in endometrium.
Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains.
Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains.
Phenotypic and functional differences between human saphenous vein (HSVEC) and umbilical vein (HUVEC) endothelial cells.
LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling.
Trafficking machinery of NKT cells: shared and differential chemokine receptor expression among V alpha 24(+)V beta 11(+) NKT cell subsets with distinct cytokine-producing capacity.
Identity of Leu-19 (CD56) leukocyte differentiation antigen and neural cell adhesion molecule.
Identity of Leu-19 (CD56) leukocyte differentiation antigen and neural cell adhesion molecule.
Kim JM, Kwon CHD, Joh JW, Sinn DH, Choi GS, Park JB, Kang ES, Lee SK
Medical principles and practice : international journal of the Kuwait University, Health Science Centre 2017;26(3):221-228
Medical principles and practice : international journal of the Kuwait University, Health Science Centre 2017;26(3):221-228
LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells.
Lu XJ, Chen Q, Rong YJ, Yang GJ, Li CH, Xu NY, Yu CH, Wang HY, Zhang S, Shi YH, Chen J
Nature communications 2016 Sep 6;7:12719
Nature communications 2016 Sep 6;7:12719
Effect of Anti-IL-15 Administration on T Cell and NK Cell Homeostasis in Rhesus Macaques.
DeGottardi MQ, Okoye AA, Vaidya M, Talla A, Konfe AL, Reyes MD, Clock JA, Duell DM, Legasse AW, Sabnis A, Park BS, Axthelm MK, Estes JD, Reiman KA, Sekaly RP, Picker LJ
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1183-98
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1183-98
Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1.
Grieco AJ, Billett HH, Green NS, Driscoll MC, Bouhassira EE
PloS one 2015;10(6):e0129431
PloS one 2015;10(6):e0129431
New IDH1 mutant inhibitors for treatment of acute myeloid leukemia.
Okoye-Okafor UC, Bartholdy B, Cartier J, Gao EN, Pietrak B, Rendina AR, Rominger C, Quinn C, Smallwood A, Wiggall KJ, Reif AJ, Schmidt SJ, Qi H, Zhao H, Joberty G, Faelth-Savitski M, Bantscheff M, Drewes G, Duraiswami C, Brady P, Groy A, Narayanagari SR, Antony-Debre I, Mitchell K, Wang HR, Kao YR, Christopeit M, Carvajal L, Barreyro L, Paietta E, Makishima H, Will B, Concha N, Adams ND, Schwartz B, McCabe MT, Maciejewski J, Verma A, Steidl U
Nature chemical biology 2015 Nov;11(11):878-86
Nature chemical biology 2015 Nov;11(11):878-86
Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.
Van Eyck L, De Somer L, Pombal D, Bornschein S, Frans G, Humblet-Baron S, Moens L, de Zegher F, Bossuyt X, Wouters C, Liston A
Arthritis & rheumatology (Hoboken, N.J.) 2015 Jun;67(6):1592-7
Arthritis & rheumatology (Hoboken, N.J.) 2015 Jun;67(6):1592-7
Impaired Th17 polarization of phenotypically naive CD4(+) T-cells during chronic HIV-1 infection and potential restoration with early ART.
DaFonseca S, Niessl J, Pouvreau S, Wacleche VS, Gosselin A, Cleret-Buhot A, Bernard N, Tremblay C, Jenabian MA, Routy JP, Ancuta P
Retrovirology 2015 Apr 30;12:38
Retrovirology 2015 Apr 30;12:38
Grb10 deletion enhances muscle cell proliferation, differentiation and GLUT4 plasma membrane translocation.
Mokbel N, Hoffman NJ, Girgis CM, Small L, Turner N, Daly RJ, Cooney GJ, Holt LJ
Journal of cellular physiology 2014 Nov;229(11):1753-64
Journal of cellular physiology 2014 Nov;229(11):1753-64
The endogenous danger signals HSP70 and MICA cooperate in the activation of cytotoxic effector functions of NK cells.
Elsner L, Flügge PF, Lozano J, Muppala V, Eiz-Vesper B, Demiroglu SY, Malzahn D, Herrmann T, Brunner E, Bickeböller H, Multhoff G, Walter L, Dressel R
Journal of cellular and molecular medicine 2010 Apr;14(4):992-1002
Journal of cellular and molecular medicine 2010 Apr;14(4):992-1002
Adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells involves CD44.
Wagner W, Wein F, Roderburg C, Saffrich R, Diehlmann A, Eckstein V, Ho AD
Cells, tissues, organs 2008;188(1-2):160-9
Cells, tissues, organs 2008;188(1-2):160-9
A new protocol for multiple inhalation of IFN-gamma successfully treats MDR-TB: a case study.
Grahmann PR, Braun RK
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease 2008 Jun;12(6):636-44
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease 2008 Jun;12(6):636-44
Unique characteristics of NK cells throughout the human female reproductive tract.
Mselle TF, Meadows SK, Eriksson M, Smith JM, Shen L, Wira CR, Sentman CL
Clinical immunology (Orlando, Fla.) 2007 Jul;124(1):69-76
Clinical immunology (Orlando, Fla.) 2007 Jul;124(1):69-76
Isolation of large numbers of mesenchymal stem cells from the washings of bone marrow collection bags: characterization of fresh mesenchymal stem cells.
Mageed AS, Pietryga DW, DeHeer DH, West RA
Transplantation 2007 Apr 27;83(8):1019-26
Transplantation 2007 Apr 27;83(8):1019-26
TLRs mediate IFN-gamma production by human uterine NK cells in endometrium.
Eriksson M, Meadows SK, Basu S, Mselle TF, Wira CR, Sentman CL
Journal of immunology (Baltimore, Md. : 1950) 2006 May 15;176(10):6219-24
Journal of immunology (Baltimore, Md. : 1950) 2006 May 15;176(10):6219-24
Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains.
Steinberger P, Majdic O, Derdak SV, Pfistershammer K, Kirchberger S, Klauser C, Zlabinger G, Pickl WF, Stöckl J, Knapp W
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 15;172(4):2352-9
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 15;172(4):2352-9
Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains.
Steinberger P, Majdic O, Derdak SV, Pfistershammer K, Kirchberger S, Klauser C, Zlabinger G, Pickl WF, Stöckl J, Knapp W
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 15;172(4):2352-9
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 15;172(4):2352-9
Phenotypic and functional differences between human saphenous vein (HSVEC) and umbilical vein (HUVEC) endothelial cells.
Tan PH, Chan C, Xue SA, Dong R, Ananthesayanan B, Manunta M, Kerouedan C, Cheshire NJ, Wolfe JH, Haskard DO, Taylor KM, George AJ
Atherosclerosis 2004 Apr;173(2):171-83
Atherosclerosis 2004 Apr;173(2):171-83
LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling.
Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V
The Journal of experimental medicine 2003 Nov 17;198(10):1453-62
The Journal of experimental medicine 2003 Nov 17;198(10):1453-62
Trafficking machinery of NKT cells: shared and differential chemokine receptor expression among V alpha 24(+)V beta 11(+) NKT cell subsets with distinct cytokine-producing capacity.
Kim CH, Johnston B, Butcher EC
Blood 2002 Jul 1;100(1):11-6
Blood 2002 Jul 1;100(1):11-6
Identity of Leu-19 (CD56) leukocyte differentiation antigen and neural cell adhesion molecule.
Lanier LL, Testi R, Bindl J, Phillips JH
The Journal of experimental medicine 1989 Jun 1;169(6):2233-8
The Journal of experimental medicine 1989 Jun 1;169(6):2233-8
Identity of Leu-19 (CD56) leukocyte differentiation antigen and neural cell adhesion molecule.
Lanier LL, Testi R, Bindl J, Phillips JH
The Journal of experimental medicine 1989 Jun 1;169(6):2233-8
The Journal of experimental medicine 1989 Jun 1;169(6):2233-8
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CD56 was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD56 Monoclonal Antibody (MEM-188) (Product # MA1-19129) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Plasma membrane localization. Panel e represents HepG2. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD56 using a monoclonal antibody (Product # MA1-19129).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis (surface staining) of human peripheral blood lymphocytes with anti-CD56 (MEM-188) purified Monoclonal antibody (Product # MA1-19129); GAM-APC.
