MA1-35249

antibody from Invitrogen Antibodies

Targeting: NCAM1 CD56, NCAM

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA1-35249

Provider

Invitrogen Antibodies

Product name

CD56 Monoclonal Antibody (123C3)

Antibody type

Monoclonal

Antigen

Other

Description

The antibody recognizes a transmembrane glycoprotein of 140 and 180 kD which has been identified as NCAM (Neural Cell Adhesion Module). At the international Workshop on SCLC antibodies 123C3 has been categorized as cluster 1 antibody. All cells in small cell carcinomas and carcinoids of the lung are strongly positive for 123C3. A minority of cases of other major types of lung carcinoma are sometimes positive as well: however this positivity is generally weak and focal. Adenoid cystic carcinomas of bronchial glands are strongly positive. Neuroblastoma's and Wilms tumors are usually also staining strongly positive. In non-small lung cell carcinomas, 123C3 staining has been associated with more advanced stage and a decreased survival after surgery. Furthermore, this antibody can be used to support diagnosis of lymphoma or to detect residual disease for cases of CD56 positive T/NK -cell lymphoma in which the neoplastic lymphoid cells are small and show minimal atypia, especially in small biopsies.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

123C3

Vial size

1 mL

Concentration

0.1 mg/mL

Storage

4° C

References

Submitted references

Usefulness of the octreotide test in Japanese patients for predicting the presence/absence of somatostatin receptor 2 expression in insulinomas.

Nakamura A, Mitsuhashi T, Takano Y, Miyoshi H, Kameda H, Nomoto H, Nagai S, Hatanaka Y, Shimizu C, Terauchi Y, Atsumi T
Endocrine journal 2016;63(2):135-42

---

Role of cd56 and e-cadherin expression in the differential diagnosis of papillary thyroid carcinoma and suspected follicular-patterned lesions of the thyroid: the prognostic importance of e-cadherin.

Ceyran AB, Şenol S, Şimşek BÇ, Sağıroğlu J, Aydın A
International journal of clinical and experimental pathology 2015;8(4):3670-80

---

Variable Expression of Neural Cell Adhesion Molecule Isoforms in Renal Tissue: Possible Role in Incipient Renal Fibrosis.

Marković-Lipkovski J, Životić M, Müller CA, Tampe B, Ćirović S, Vještica J, Tomanović N, Zeisberg M, Müller GA
PloS one 2015;10(9):e0137028

---

CD30(+) extranodal natural killer/T-cell lymphoma mimicking phlegmonous myositis: A case report.

Yang YJ, Li YX, Liu YB, Yang M, Liu K
Oncology letters 2014 May;7(5):1419-1421

---

A Case of Distal Epithelioid Sarcoma of the Thumb Expressing Podoplanin, TLE1 and Ca 125.

Karagkounis G, Argyrakos T, Charkiolakis G, Castana O, Rontogianni D
Case reports in pathology 2013;2013:312786

---

Serous effusion cytology of extranodal natural killer/T-cell lymphoma.

Su XY, Huang J, Jiang Y, Tang Y, Li GD, Liu WP
Cytopathology : official journal of the British Society for Clinical Cytology 2012 Apr;23(2):96-102

---

Extraosseous (extramedullary) plasmacytomas: a clinicopathologic and immunophenotypic study of 32 Chinese cases.

Zuo Z, Tang Y, Bi CF, Zhang WY, Zhao S, Wang XQ, Yang QP, Zou LQ, Liu WP
Diagnostic pathology 2011 Dec 19;6:123

---

Subcutaneous panniculitis-like T-cell lymphoma: a clinical and pathologic study of 14 korean patients.

Lee DW, Yang JH, Lee SM, Won CH, Chang S, Lee MW, Choi JH, Moon KC
Annals of dermatology 2011 Aug;23(3):329-37

---

Primary central nervous system peripheral T-cell lymphoma in a child.

Gualco G, Wludarski S, Hayashi-Silva L, Medeiros Filho P, Veras G, Bacchi CE
Fetal and pediatric pathology 2010;29(4):224-30

---

Primary plasmacytoma of the cervix in a 21-year-old female patient.

Schor AP, Moraes MP, Bisson FW, Bisson MA, Luiz OM, Bacchi CE
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2010 May;29(3):290-3

---

Atypical carcinoid with prominent mucinous stroma: a hitherto unreported variant of pulmonary neuroendocrine tumor.

Nannini N, Bertolini F, Cavazza A, Casali C, Mengoli MC, Rossi G
Endocrine pathology 2010 Jun;21(2):120-4

---

Goblet cell carcinoid of the appendix: a clinicopathological and immunohistochemical study of 26 cases from southwest china.

Yong Jiang, Huawei Liu, Hu Long, Yingying Yang, Dianying Liao, Xiuhui Zhang
International journal of surgical pathology 2010 Dec;18(6):488-92

---

Composite lymphoma: EBV-positive classic Hodgkin lymphoma and peripheral T-cell lymphoma: a case report.

Gualco G, Chioato L, Van Den Berg A, Weiss LM, Bacchi CE
Applied immunohistochemistry & molecular morphology : AIMM 2009 Jan;17(1):72-6

---

Composite lymphoma: EBV-positive classic Hodgkin lymphoma and peripheral T-cell lymphoma: a case report.

Gualco G, Chioato L, Van Den Berg A, Weiss LM, Bacchi CE
Applied immunohistochemistry & molecular morphology : AIMM 2009 Jan;17(1):72-6

---

EGFR and K-ras mutations along the spectrum of pulmonary epithelial tumors of the lung and elaboration of a combined clinicopathologic and molecular scoring system to predict clinical responsiveness to EGFR inhibitors.

Sartori G, Cavazza A, Sgambato A, Marchioni A, Barbieri F, Longo L, Bavieri M, Murer B, Meschiari E, Tamberi S, Cadioli A, Luppi F, Migaldi M, Rossi G
American journal of clinical pathology 2009 Apr;131(4):478-89

---

The prognostic role of c-kit protein expression in resected large cell neuroendocrine carcinoma of the lung.

Casali C, Stefani A, Rossi G, Migaldi M, Bettelli S, Parise A, Morandi U
The Annals of thoracic surgery 2004 Jan;77(1):247-52; discussion 252-3

---

The prognostic role of c-kit protein expression in resected large cell neuroendocrine carcinoma of the lung.

Casali C, Stefani A, Rossi G, Migaldi M, Bettelli S, Parise A, Morandi U
The Annals of thoracic surgery 2004 Jan;77(1):247-52; discussion 252-3

---

TTF-1, cytokeratin 7, 34betaE12, and CD56/NCAM immunostaining in the subclassification of large cell carcinomas of the lung.

Rossi G, Marchioni A, Milani M, Scotti R, Foroni M, Cesinaro A, Longo L, Migaldi M, Cavazza A
American journal of clinical pathology 2004 Dec;122(6):884-93

---

Desmoplastic and spindle cell melanomas express protein markers of the neural crest but not of later committed stages of Schwann cell differentiation.

Huttenbach Y, Prieto VG, Reed JA
Journal of cutaneous pathology 2002 Oct;29(9):562-8

---

Desmoplastic and spindle cell melanomas express protein markers of the neural crest but not of later committed stages of Schwann cell differentiation.

Huttenbach Y, Prieto VG, Reed JA
Journal of cutaneous pathology 2002 Oct;29(9):562-8

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CD56 was performed using 70% confluent log phase IMR-32. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD56 Monoclonal Antibody (Product # MA1-35249) at 1:20 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Red) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Blue) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing plasma membrane localization. Panel e represents negative cell line, HepG2, with no expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.