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LearnMA5-31778
antibody from Invitrogen Antibodies
Targeting: IL1RAPL1
IL1R8, IL1RAPL, MRX10, MRX21, MRX34, OPHN4, TIGIRR-2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-31778 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL1RAPL1 Monoclonal Antibody (3E3D12)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-31778 has been tested in indirect ELISA.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3E3D12
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL1RAPL1 in A431 (1), SK-Hep-1 (2), and HL-7702 (3) cell lysate. Samples were incubated with IL1RAPL1 monoclonal antibody (Product # MA5-31778) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL1RAPL1 in human IL1RAPL1 recombinant protein. Sample was incubated with IL1RAPL1 monoclonal antibody (Product # MA5-31778) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL1RAPL1 in HEK293 (1) and IL1RAPL1 cell lysate. Samples were incubated with IL1RAPL1 monoclonal antibody (Product # MA5-31778) using a dilution of 1:500-1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of IL1RAPL1 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with IL1RAPL1 monoclonal antibody (Product # MA5-31778) using a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of IL1RAPL1 in Hela cells (green). Sample was incubated with IL1RAPL1 monoclonal antibody (Product # MA5-31778) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IL1RAPL1 in HeLa cells (green). Sample was incubated with IL1RAPL1 monoclonal antibody (Product # MA5-31778) using a dilution of 1:200-1:400 followed by negative control (red).
