MA5-17164

antibody from Invitrogen Antibodies

Targeting: RAP1A KREV-1, SMGP21

ELISA

Antibody data

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Validation data

Product number: MA5-17164 - Provider product page
Provider: Invitrogen Antibodies
Product name: RAP1A Monoclonal Antibody (5F8)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-17164 targets RAP1A in FACS, indirect ELISA, and WB applications and shows reactivity with Human samples. The MA5-17164 immunogen is purified recombinant fragment of human RAP1A expressed in E. Coli. MA5-17164 detects RAP1A which has a predicted molecular weight of approximately 21kDa.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 5F8
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RAP1A protein structure - MA5-17164 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RAP1A using a RAP1A monoclonal antibody (Product # MA5-17164) against a human RAP1A (AA: 28-180) recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RAP1A using RAP1A monoclonal antibody (Product # MA5-17164) in HEK293 (1) and RAP1A (AA: 28-180) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-RAP1A Monoclonal Antibody (5F8) (Product # MA5-17164) and 20 kDa band corresponding to RAP1A was observed across the cell lines tested except C2C12 in which the expression was observed upon myoblast differentiation. Whole cell lysates (30ug lysate) of C2C12 (Lane 1), C2C12 differentiated to Myoblasts (Lane2), HT-29 (Lane 3), MDA-MB-231 (Lane 4), MCF 10A (Lane 5), A-431 (Lane 6), HeLa (Lane 7) and A-375 (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RAP1A using a RAP1A monoclonal antibody (Product # MA5-17164) against a human RAP1A (AA: 28-180) recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RAP1A using RAP1A monoclonal antibody (Product # MA5-17164) in HEK293 (1) and RAP1A (AA: 28-180) human IgG Fc transfected HEK293 (2) cell lysate.