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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-65 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-65, RRID:AB_632321
- Product name
- Anti-RAP1A
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Polytrauma in the elderly: predictors of the cause and time of death.
Downregulation of Rap1GAP in human tumor cells alters cell/matrix and cell/cell adhesion.
Vascular effects of a low-carbohydrate high-protein diet.
PKA and Epac cooperate to augment bradykinin-induced interleukin-8 release from human airway smooth muscle cells.
Direct spatial control of Epac1 by cyclic AMP.
Sleep-dependent gene expression in the hippocampus and prefrontal cortex following long-term potentiation.
Extracellular calcium increases bisphosphonate-induced growth inhibition of breast cancer cells.
Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct.
Combinatorial interaction between CCM pathway genes precipitates hemorrhagic stroke.
Thrombospondin-1 stimulates platelet aggregation by blocking the antithrombotic activity of nitric oxide/cGMP signaling.
Inhibitors of protein geranylgeranyltransferase I and Rab geranylgeranyltransferase identified from a library of allenoate-derived compounds.
Inhibition of HMGcoA reductase by atorvastatin prevents and reverses MYC-induced lymphomagenesis.
GGTase-I deficiency reduces tumor formation and improves survival in mice with K-RAS-induced lung cancer.
Preemptive HMG-CoA reductase inhibition provides graft-versus-host disease protection by Th-2 polarization while sparing graft-versus-leukemia activity.
HIV protease inhibitors block the zinc metalloproteinase ZMPSTE24 and lead to an accumulation of prelamin A in cells.
Impaired fertility and spermiogenetic disorders with loss of cell adhesion in male mice expressing an interfering Rap1 mutant.
Zoledronic acid treatment impairs protein geranyl-geranylation for biological effects in prostatic cells.
Rap1A-deficient T and B cells show impaired integrin-mediated cell adhesion.
Isoprenoids determine Th1/Th2 fate in pathogenic T cells, providing a mechanism of modulation of autoimmunity by atorvastatin.
High-throughput identification of IMCD proteins using LC-MS/MS.
Histone deacetylase inhibitor FK228 suppresses the Ras–MAP kinase signaling pathway by upregulating Rap1 and induces apoptosis in malignant melanoma
Clement ND, Tennant C, Muwanga C
Scandinavian journal of trauma, resuscitation and emergency medicine 2010 May 13;18:26
Scandinavian journal of trauma, resuscitation and emergency medicine 2010 May 13;18:26
Downregulation of Rap1GAP in human tumor cells alters cell/matrix and cell/cell adhesion.
Tsygankova OM, Ma C, Tang W, Korch C, Feldman MD, Lv Y, Brose MS, Meinkoth JL
Molecular and cellular biology 2010 Jul;30(13):3262-74
Molecular and cellular biology 2010 Jul;30(13):3262-74
Vascular effects of a low-carbohydrate high-protein diet.
Foo SY, Heller ER, Wykrzykowska J, Sullivan CJ, Manning-Tobin JJ, Moore KJ, Gerszten RE, Rosenzweig A
Proceedings of the National Academy of Sciences of the United States of America 2009 Sep 8;106(36):15418-23
Proceedings of the National Academy of Sciences of the United States of America 2009 Sep 8;106(36):15418-23
PKA and Epac cooperate to augment bradykinin-induced interleukin-8 release from human airway smooth muscle cells.
Roscioni SS, Kistemaker LE, Menzen MH, Elzinga CR, Gosens R, Halayko AJ, Meurs H, Schmidt M
Respiratory research 2009 Sep 29;10(1):88
Respiratory research 2009 Sep 29;10(1):88
Direct spatial control of Epac1 by cyclic AMP.
Ponsioen B, Gloerich M, Ritsma L, Rehmann H, Bos JL, Jalink K
Molecular and cellular biology 2009 May;29(10):2521-31
Molecular and cellular biology 2009 May;29(10):2521-31
Sleep-dependent gene expression in the hippocampus and prefrontal cortex following long-term potentiation.
Romcy-Pereira RN, Erraji-Benchekroun L, Smyrniotopoulos P, Ogawa S, Mello CV, Sibille E, Pavlides C
Physiology & behavior 2009 Aug 4;98(1-2):44-52
Physiology & behavior 2009 Aug 4;98(1-2):44-52
Extracellular calcium increases bisphosphonate-induced growth inhibition of breast cancer cells.
Journé F, Kheddoumi N, Chaboteaux C, Duvillier H, Laurent G, Body JJ
Breast cancer research : BCR 2008;10(1):R4
Breast cancer research : BCR 2008;10(1):R4
Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct.
Yu MJ, Pisitkun T, Wang G, Aranda JF, Gonzales PA, Tchapyjnikov D, Shen RF, Alonso MA, Knepper MA
American journal of physiology. Cell physiology 2008 Sep;295(3):C661-78
American journal of physiology. Cell physiology 2008 Sep;295(3):C661-78
Combinatorial interaction between CCM pathway genes precipitates hemorrhagic stroke.
Gore AV, Lampugnani MG, Dye L, Dejana E, Weinstein BM
Disease models & mechanisms 2008 Nov-Dec;1(4-5):275-81
Disease models & mechanisms 2008 Nov-Dec;1(4-5):275-81
Thrombospondin-1 stimulates platelet aggregation by blocking the antithrombotic activity of nitric oxide/cGMP signaling.
Isenberg JS, Romeo MJ, Yu C, Yu CK, Nghiem K, Monsale J, Rick ME, Wink DA, Frazier WA, Roberts DD
Blood 2008 Jan 15;111(2):613-23
Blood 2008 Jan 15;111(2):613-23
Inhibitors of protein geranylgeranyltransferase I and Rab geranylgeranyltransferase identified from a library of allenoate-derived compounds.
Watanabe M, Fiji HD, Guo L, Chan L, Kinderman SS, Slamon DJ, Kwon O, Tamanoi F
The Journal of biological chemistry 2008 Apr 11;283(15):9571-9
The Journal of biological chemistry 2008 Apr 11;283(15):9571-9
Inhibition of HMGcoA reductase by atorvastatin prevents and reverses MYC-induced lymphomagenesis.
Shachaf CM, Perez OD, Youssef S, Fan AC, Elchuri S, Goldstein MJ, Shirer AE, Sharpe O, Chen J, Mitchell DJ, Chang M, Nolan GP, Steinman L, Felsher DW
Blood 2007 Oct 1;110(7):2674-84
Blood 2007 Oct 1;110(7):2674-84
GGTase-I deficiency reduces tumor formation and improves survival in mice with K-RAS-induced lung cancer.
Sjogren AK, Andersson KM, Liu M, Cutts BA, Karlsson C, Wahlstrom AM, Dalin M, Weinbaum C, Casey PJ, Tarkowski A, Swolin B, Young SG, Bergo MO
The Journal of clinical investigation 2007 May;117(5):1294-304
The Journal of clinical investigation 2007 May;117(5):1294-304
Preemptive HMG-CoA reductase inhibition provides graft-versus-host disease protection by Th-2 polarization while sparing graft-versus-leukemia activity.
Zeiser R, Youssef S, Baker J, Kambham N, Steinman L, Negrin RS
Blood 2007 Dec 15;110(13):4588-98
Blood 2007 Dec 15;110(13):4588-98
HIV protease inhibitors block the zinc metalloproteinase ZMPSTE24 and lead to an accumulation of prelamin A in cells.
Coffinier C, Hudon SE, Farber EA, Chang SY, Hrycyna CA, Young SG, Fong LG
Proceedings of the National Academy of Sciences of the United States of America 2007 Aug 14;104(33):13432-7
Proceedings of the National Academy of Sciences of the United States of America 2007 Aug 14;104(33):13432-7
Impaired fertility and spermiogenetic disorders with loss of cell adhesion in male mice expressing an interfering Rap1 mutant.
Aivatiadou E, Mattei E, Ceriani M, Tilia L, Berruti G
Molecular biology of the cell 2007 Apr;18(4):1530-42
Molecular biology of the cell 2007 Apr;18(4):1530-42
Zoledronic acid treatment impairs protein geranyl-geranylation for biological effects in prostatic cells.
Goffinet M, Thoulouzan M, Pradines A, Lajoie-Mazenc I, Weinbaum C, Faye JC, Séronie-Vivien S
BMC cancer 2006 Mar 15;6:60
BMC cancer 2006 Mar 15;6:60
Rap1A-deficient T and B cells show impaired integrin-mediated cell adhesion.
Duchniewicz M, Zemojtel T, Kolanczyk M, Grossmann S, Scheele JS, Zwartkruis FJ
Molecular and cellular biology 2006 Jan;26(2):643-53
Molecular and cellular biology 2006 Jan;26(2):643-53
Isoprenoids determine Th1/Th2 fate in pathogenic T cells, providing a mechanism of modulation of autoimmunity by atorvastatin.
Dunn SE, Youssef S, Goldstein MJ, Prod'homme T, Weber MS, Zamvil SS, Steinman L
The Journal of experimental medicine 2006 Feb 20;203(2):401-12
The Journal of experimental medicine 2006 Feb 20;203(2):401-12
High-throughput identification of IMCD proteins using LC-MS/MS.
Pisitkun T, Bieniek J, Tchapyjnikov D, Wang G, Wu WW, Shen RF, Knepper MA
Physiological genomics 2006 Apr 13;25(2):263-76
Physiological genomics 2006 Apr 13;25(2):263-76
Histone deacetylase inhibitor FK228 suppresses the Ras–MAP kinase signaling pathway by upregulating Rap1 and induces apoptosis in malignant melanoma
Y Kobayashi, M Ohtsuki, T Murakami, T Kobayashi, K Sutheesophon, H Kitayama, Y Kano, E Kusano, H Nakagawa, Y Furukawa
Oncogene 2005 Sep;25(4):512-524
Oncogene 2005 Sep;25(4):512-524
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 21
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in Islet cells and moderate cytoplasmic staining in exocrine glands.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
