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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX31146 - Provider product page
- Provider
- GeneTex
- Product name
- Rap1 antibody [1D2-1C64]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of various cell lysates (25 μg per lane) using Rap1 antibody [1D2-1C64] at a dilution of 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HeLa cells using Rap1 antibody [1D2-1C64] at a dilution of 1:100 (green). Nuclei (blue) were stained with Hoechst 33342.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of C2C12 cells with (right) or without (left) Rap1 antibody [1D2-1C64] at a dilution of 1:20 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IP analysis was performed with 750ug of NIH3T3 lysate and 2ug of Rap1 antibody [1D2-1C64]. 25ug of NIH3T3 lysate (left) and the precipitate were detected by the same antibody at a dilution of 1:500 with EasyBlot as secondary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human kidney tissue with (right) or without (left) Rap1 antibody [1D2-1C64] at a dilution of 1:200. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.
