H00005906-M01

antibody from Abnova Corporation

Targeting: RAP1A KREV-1, SMGP21

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005906-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005906-M01, RRID:AB_606911

Product name

RAP1A monoclonal antibody (M01), clone 3E1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant RAP1A.

Antigen sequence

MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPT
IEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDL
YMKNGQGFALVYSITAQSTFNDLQDLREQILRVKD
TEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCN
CAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKK
PKKKSCLLL


Isotype

IgG

Antibody clone number

3E1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Overexpression of Rap-1A indicates a poor prognosis for oral cavity squamous cell carcinoma and promotes tumor cell invasion via Aurora-A modulation.

Chen CH, Chuang HC, Huang CC, Fang FM, Huang HY, Tsai HT, Su LJ, Shiu LY, Leu S, Chien CY
The American journal of pathology 2013 Feb;182(2):516-28

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RAP1A is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between RASGRP4 and RAP1A. HeLa cells were stained with anti-RASGRP4 rabbit purified polyclonal 1:1200 and anti-RAP1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)