MA5-23812

antibody from Invitrogen Antibodies

Targeting: TGFB3 ARVD, ARVD1

Western blot (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23812

Provider

Invitrogen Antibodies

Product name

TGF beta-3 Monoclonal Antibody (20724)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In Western blots, less than 25% cross-reactivity with recombinant human (rh) TGF- beta 1.2 and rhTGF-beta 2 is observed, and less than 2% cross-reactivity with recombinant amphibian TGF-beta 5 and recombinant human TGF-beta 1 is observed. This antibody neutralizes the biological activity of TGF-beta 3 but not TGF-beta 1, TGF-beta 2, or TGF-beta 5. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

20724

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of TGF beta-3 in HT‚2 mouse T cell line. Samples were incubated in TGF beta-3 monoclonal antibody (Product # MA5-23812). Recombinant Human TGF‚β3 inhibits Recombinant Mouse IL‚4 induced proliferation in the HT‚2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‚4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‚β3 (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF‚β3 Monoclonal Antibody. The ND50 is typically 0.1-0.3 µg/mL.