Explore
Validate
Learn
Western blot
Immunohistochemistry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-23812 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TGF beta-3 Monoclonal Antibody (20724)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, less than 25% cross-reactivity with recombinant human (rh) TGF- beta 1.2 and rhTGF-beta 2 is observed, and less than 2% cross-reactivity with recombinant amphibian TGF-beta 5 and recombinant human TGF-beta 1 is observed. This antibody neutralizes the biological activity of TGF-beta 3 but not TGF-beta 1, TGF-beta 2, or TGF-beta 5. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 20724
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TGF beta-3 in immersion fixed paraffin-embedded sections of human breast cancer tissue. Samples were incubated in TGF beta-3 monoclonal antibody (Product # MA5-23812) using a dilution of 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of TGF beta-3 in HT‚2 mouse T cell line. Samples were incubated in TGF beta-3 monoclonal antibody (Product # MA5-23812). Recombinant Human TGF‚β3 inhibits Recombinant Mouse IL‚4 induced proliferation in the HT‚2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‚4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‚β3 (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF‚β3 Monoclonal Antibody. The ND50 is typically 0.1-0.3 µg/mL.
