PA5-32630

antibody from Invitrogen Antibodies

Targeting: TGFB3 ARVD, ARVD1

Antibody data

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Validation data

Product number: PA5-32630 - Provider product page
Provider: Invitrogen Antibodies
Product name: TGF beta-3 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody is predicted to react with chicken, canine, mouse, porcine and rat based on sequence homology.

TGFB3 protein structure - PA5-32630 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Transforming growth factor beta-3 pro protein was achieved by transfecting MCF7 with Transforming growth factor beta-3 pro protein specific siRNAs (Silencer® select Product # s14064, s14063). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Transforming growth factor beta-3 pro protein knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with TGF beta-3 Polyclonal Antibody (Product # PA5-32630, 1:1000 dilution ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Transforming growth factor beta-3 pro protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-TGF beta-3 Polyclonal Antibody (Product # PA5-32630) and a 55 kDa band corresponding to Transforming growth factor beta-3 pro protein was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of SK-BR-3 (Lane 1), U-87 MG (Lane 2), HeLa (Lane 3), MCF7 (Lane 4), SK-O-V3 (Lane 5), HEL 92.1.7 (Lane 6), Daudi (Lane 7), Mouse Ovary (Lane 8), Rat Ovary (Lane 9), Mouse Heart (Lane 10), Rat Heart (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).