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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-29927 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CREM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, Neuro2A, GL261, PC-12, Rat2. Predicted reactivity: Mouse (93%), Rat (91%), Dog (86%), Chicken (88%), Bovine (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.31 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Impact of the acidic environment on gene expression and functional parameters of tumors in vitro and in vivo.
Rauschner M, Lange L, Hüsing T, Reime S, Nolze A, Maschek M, Thews O, Riemann A
Journal of experimental & clinical cancer research : CR 2021 Jan 6;40(1):10
Journal of experimental & clinical cancer research : CR 2021 Jan 6;40(1):10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CREM in paraformaldehyde-fixed HeLa cells using a CREM polyclonal antibody (Product # PA5-29927) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 Change of protein levels of PER3, GLS2, CREM and TXNIP in AT1 prostate and Walker-256 mammary carcinoma cells after 24 h under acidotic (pH 6.6) and/or hypoxic (pO 2 = 1.5 mmHg) conditions. n = 4-12; (*) p < 0.05, (**) p < 0.01 vs. control; ( # ) p < 0.05, ( ## ) p < 0.01 AT1 vs. Walker-256 cells
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Protein levels of PER3, GLS2, CREM and TXNIP in AT1 prostate and Walker-256 mammary carcinoma tumors in vivo 24 h after ( a ) inducing metabolic acidosis by forcing glycolytic metabolism or ( b ) intratumoral injection of lactic acid. n = 4-18; (*) p < 0.05, (**) p < 0.01 vs. control; ( # ) p < 0.05, ( ## ) p < 0.01 AT1 vs. Walker-256 cells
