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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- HPA001818 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA001818, RRID:AB_1078566
- Product name
- Anti-CREM
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human CREM, Gene description: cAMP responsive element modulator, Alternative Gene Names: hCREM-2, Validated applications: WB, IHC, ChIP, Uniprot ID: Q03060, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Partial rejuvenation of the spermatogonial stem cell niche after gender-affirming hormone therapy in trans women
Delgouffe E, Silva S, Chalmel F, Cools W, Raets C, Tilleman K, T'Sjoen G, Baert Y, Goossens E
eLife 2025;13
eLife 2025;13
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human testis and fallopian tube tissues using HPA001818 antibody. Corresponding CREM RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- ChIP-Exo-Seq composite graph for Anti-CREM (HPA001818, Lot 000050544) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
