Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- AF368 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Flt-3/Flk-2/CD135 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Flt-3 when phosphorylated at Y591.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Phospho-Flt-3/Flk-2 (Y591) by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-Flt-3/Flk-2 (Y591) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF368), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-Flt-3/Flk-2 (Y591) at approximately 160 and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Phospho-Flt-3/Flk-2 (Y591) by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 µM Pervanadate (PV) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Flt-3/Flk-2 (Y591) at approximately 166 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-Flt-3/Flk-2 (Y591) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF368). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.