PA5-105885

antibody from Invitrogen Antibodies

Targeting: FLT3 CD135, FLK2, STK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-105885

Provider

Invitrogen Antibodies

Product name

Phospho-FLT3 (Tyr589) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of Flt3 / CD135 only when phosphorylated at Tyr589.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-FLT3 (Tyr589) in insulin treated 293 cell lysate (absence (-) or presence (+) of non-phospho and phospho peptide). Samples were incubated with Phospho-FLT3 (Tyr589) polyclonal antibody (Product # PA5-105885).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-FLT3 (Tyr589) in COS7 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with Phospho-FLT3 (Tyr589) polyclonal antibody (Product # PA5-105885) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-FLT3 (Tyr589) in COS7 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with Phospho-FLT3 (Tyr589) polyclonal antibody (Product # PA5-105885) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.