Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [27]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14525-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14525-1-AP, RRID:AB_2205881
- Product name
- TOP3A antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated TOP3A antibody (Cat. #14525-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IP, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Mitochondrial DNA replication stress triggers a pro-inflammatory endosomal pathway of nucleoid disposal.
BLM and BRCA1-BARD1 coordinate complementary mechanisms of joint DNA molecule resolution.
Pathological variants in TOP3A cause distinct disorders of mitochondrial and nuclear genome stability.
Resolution of sequence divergence for repeat-mediated deletions shows a polarity that is mediated by MLH1.
RIF1 suppresses the formation of single-stranded ultrafine anaphase bridges via protein phosphatase 1.
Centromeres as universal hotspots of DNA breakage, driving RAD51-mediated recombination during quiescence.
Replication-associated formation and repair of human topoisomerase IIIα cleavage complexes.
The human mitochondrial genome contains a second light strand promoter.
TOP3A amplification and ATRX inactivation are mutually exclusive events in pediatric osteosarcomas using ALT.
Top3α is the replicative topoisomerase in mitochondrial DNA replication.
Predominant cellular mitochondrial dysfunction in the TOP3A gene-caused Bloom syndrome-like disorder.
The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.
PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks.
The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks.
Nuclear envelope tethering inhibits the formation of ALT-associated PML bodies in ALT cells.
FANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loops.
Mutations in TOP3A Cause a Bloom Syndrome-like Disorder.
Ciprofloxacin impairs mitochondrial DNA replication initiation through inhibition of Topoisomerase 2.
HERC2 Facilitates BLM and WRN Helicase Complex Interaction with RPA to Suppress G-Quadruplex DNA.
Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.
Mutations in TOP3A Cause a Bloom Syndrome-like Disorder.
BLM helicase regulates DNA repair by counteracting RAD51 loading at DNA double-strand break sites.
RECQ-like helicases Sgs1 and BLM regulate R-loop-associated genome instability.
FANCD2, FANCJ and BRCA2 cooperate to promote replication fork recovery independently of the Fanconi Anemia core complex.
TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation.
FANCD2-controlled chromatin access of the Fanconi-associated nuclease FAN1 is crucial for the recovery of stalled replication forks.
FANCD2 regulates BLM complex functions independently of FANCI to promote replication fork recovery.
Newman LE, Weiser Novak S, Rojas GR, Tadepalle N, Schiavon CR, Grotjahn DA, Towers CG, Tremblay MÈ, Donnelly MP, Ghosh S, Medina M, Rocha S, Rodriguez-Enriquez R, Chevez JA, Lemersal I, Manor U, Shadel GS
Nature cell biology 2024 Feb;26(2):194-206
Nature cell biology 2024 Feb;26(2):194-206
BLM and BRCA1-BARD1 coordinate complementary mechanisms of joint DNA molecule resolution.
Tsukada K, Jones SE, Bannister J, Durin MA, Vendrell I, Fawkes M, Fischer R, Kessler BM, Chapman JR, Blackford AN
Molecular cell 2024 Feb 15;84(4):640-658.e10
Molecular cell 2024 Feb 15;84(4):640-658.e10
Pathological variants in TOP3A cause distinct disorders of mitochondrial and nuclear genome stability.
Erdinc D, Rodríguez-Luis A, Fassad MR, Mackenzie S, Watson CM, Valenzuela S, Xie X, Menger KE, Sergeant K, Craig K, Hopton S, Falkous G, Genomics England Research Consortium, Poulton J, Garcia-Moreno H, Giunti P, de Moura Aschoff CA, Morales Saute JA, Kirby AJ, Toro C, Wolfe L, Novacic D, Greenbaum L, Eliyahu A, Barel O, Anikster Y, McFarland R, Gorman GS, Schaefer AM, Gustafsson CM, Taylor RW, Falkenberg M, Nicholls TJ
EMBO molecular medicine 2023 May 8;15(5):e16775
EMBO molecular medicine 2023 May 8;15(5):e16775
Resolution of sequence divergence for repeat-mediated deletions shows a polarity that is mediated by MLH1.
Trost H, Merkell A, Lopezcolorado FW, Stark JM
Nucleic acids research 2023 Jan 25;51(2):650-667
Nucleic acids research 2023 Jan 25;51(2):650-667
RIF1 suppresses the formation of single-stranded ultrafine anaphase bridges via protein phosphatase 1.
Kong N, Liu Z, Chan YW
Cell reports 2023 Feb 28;42(2):112032
Cell reports 2023 Feb 28;42(2):112032
Centromeres as universal hotspots of DNA breakage, driving RAD51-mediated recombination during quiescence.
Saayman X, Graham E, Nathan WJ, Nussenzweig A, Esashi F
Molecular cell 2023 Feb 16;83(4):523-538.e7
Molecular cell 2023 Feb 16;83(4):523-538.e7
Replication-associated formation and repair of human topoisomerase IIIα cleavage complexes.
Saha LK, Saha S, Yang X, Huang SN, Sun Y, Jo U, Pommier Y
Nature communications 2023 Apr 6;14(1):1925
Nature communications 2023 Apr 6;14(1):1925
The human mitochondrial genome contains a second light strand promoter.
Tan BG, Mutti CD, Shi Y, Xie X, Zhu X, Silva-Pinheiro P, Menger KE, Díaz-Maldonado H, Wei W, Nicholls TJ, Chinnery PF, Minczuk M, Falkenberg M, Gustafsson CM
Molecular cell 2022 Oct 6;82(19):3646-3660.e9
Molecular cell 2022 Oct 6;82(19):3646-3660.e9
TOP3A amplification and ATRX inactivation are mutually exclusive events in pediatric osteosarcomas using ALT.
de Nonneville A, Salas S, Bertucci F, Sobinoff AP, Adélaïde J, Guille A, Finetti P, Noble JR, Churikov D, Chaffanet M, Lavit E, Pickett HA, Bouvier C, Birnbaum D, Reddel RR, Géli V
EMBO molecular medicine 2022 Oct 10;14(10):e15859
EMBO molecular medicine 2022 Oct 10;14(10):e15859
Top3α is the replicative topoisomerase in mitochondrial DNA replication.
Hangas A, Kekäläinen NJ, Potter A, Michell C, Aho KJ, Rutanen C, Spelbrink JN, Pohjoismäki JL, Goffart S
Nucleic acids research 2022 Aug 26;50(15):8733-8748
Nucleic acids research 2022 Aug 26;50(15):8733-8748
Predominant cellular mitochondrial dysfunction in the TOP3A gene-caused Bloom syndrome-like disorder.
Jiang W, Jia N, Guo C, Wen J, Wu L, Ogi T, Zhang H
Biochimica et biophysica acta. Molecular basis of disease 2021 Jun 1;1867(6):166106
Biochimica et biophysica acta. Molecular basis of disease 2021 Jun 1;1867(6):166106
The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.
Jiang M, Xie X, Zhu X, Jiang S, Milenkovic D, Misic J, Shi Y, Tandukar N, Li X, Atanassov I, Jenninger L, Hoberg E, Albarran-Gutierrez S, Szilagyi Z, Macao B, Siira SJ, Carelli V, Griffith JD, Gustafsson CM, Nicholls TJ, Filipovska A, Larsson NG, Falkenberg M
Science advances 2021 Jul;7(27)
Science advances 2021 Jul;7(27)
PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks.
González-Acosta D, Blanco-Romero E, Ubieto-Capella P, Mutreja K, Míguez S, Llanos S, García F, Muñoz J, Blanco L, Lopes M, Méndez J
The EMBO journal 2021 Jul 15;40(14):e106355
The EMBO journal 2021 Jul 15;40(14):e106355
The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks.
Shorrocks AK, Jones SE, Tsukada K, Morrow CA, Belblidia Z, Shen J, Vendrell I, Fischer R, Kessler BM, Blackford AN
Nature communications 2021 Jan 26;12(1):585
Nature communications 2021 Jan 26;12(1):585
Nuclear envelope tethering inhibits the formation of ALT-associated PML bodies in ALT cells.
Yang CW, Hsieh MH, Sun HJ, Teng SC
Aging 2021 Apr 4;13(7):10490-10516
Aging 2021 Apr 4;13(7):10490-10516
FANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loops.
Pan X, Chen Y, Biju B, Ahmed N, Kong J, Goldenberg M, Huang J, Mohan N, Klosek S, Parsa K, Guh CY, Lu R, Pickett HA, Chu HP, Zhang D
Scientific reports 2019 Dec 13;9(1):19110
Scientific reports 2019 Dec 13;9(1):19110
Mutations in TOP3A Cause a Bloom Syndrome-like Disorder.
Martin CA, Sarlós K, Logan CV, Thakur RS, Parry DA, Bizard AH, Leitch A, Cleal L, Ali NS, Al-Owain MA, Allen W, Altmüller J, Aza-Carmona M, Barakat BAY, Barraza-García J, Begtrup A, Bogliolo M, Cho MT, Cruz-Rojo J, Dhahrabi HAM, Elcioglu NH, GOSgene, Gorman GS, Jobling R, Kesterton I, Kishita Y, Kohda M, Le Quesne Stabej P, Malallah AJ, Nürnberg P, Ohtake A, Okazaki Y, Pujol R, Ramirez MJ, Revah-Politi A, Shimura M, Stevens P, Taylor RW, Turner L, Williams H, Wilson C, Yigit G, Zahavich L, Alkuraya FS, Surralles J, Iglesias A, Murayama K, Wollnik B, Dattani M, Heath KE, Hickson ID, Jackson AP
American journal of human genetics 2018 Sep 6;103(3):456
American journal of human genetics 2018 Sep 6;103(3):456
Ciprofloxacin impairs mitochondrial DNA replication initiation through inhibition of Topoisomerase 2.
Hangas A, Aasumets K, Kekäläinen NJ, Paloheinä M, Pohjoismäki JL, Gerhold JM, Goffart S
Nucleic acids research 2018 Oct 12;46(18):9625-9636
Nucleic acids research 2018 Oct 12;46(18):9625-9636
HERC2 Facilitates BLM and WRN Helicase Complex Interaction with RPA to Suppress G-Quadruplex DNA.
Wu W, Rokutanda N, Takeuchi J, Lai Y, Maruyama R, Togashi Y, Nishikawa H, Arai N, Miyoshi Y, Suzuki N, Saeki Y, Tanaka K, Ohta T
Cancer research 2018 Nov 15;78(22):6371-6385
Cancer research 2018 Nov 15;78(22):6371-6385
Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.
Nicholls TJ, Nadalutti CA, Motori E, Sommerville EW, Gorman GS, Basu S, Hoberg E, Turnbull DM, Chinnery PF, Larsson NG, Larsson E, Falkenberg M, Taylor RW, Griffith JD, Gustafsson CM
Molecular cell 2018 Jan 4;69(1):9-23.e6
Molecular cell 2018 Jan 4;69(1):9-23.e6
Mutations in TOP3A Cause a Bloom Syndrome-like Disorder.
Martin CA, Sarlós K, Logan CV, Thakur RS, Parry DA, Bizard AH, Leitch A, Cleal L, Ali NS, Al-Owain MA, Allen W, Altmüller J, Aza-Carmona M, Barakat BAY, Barraza-García J, Begtrup A, Bogliolo M, Cho MT, Cruz-Rojo J, Dhahrabi HAM, Elcioglu NH, GOSgene, Gorman GS, Jobling R, Kesterton I, Kishita Y, Kohda M, Le Quesne Stabej P, Malallah AJ, Nürnberg P, Ohtake A, Okazaki Y, Pujol R, Ramirez MJ, Revah-Politi A, Shimura M, Stevens P, Taylor RW, Turner L, Williams H, Wilson C, Yigit G, Zahavich L, Alkuraya FS, Surralles J, Iglesias A, Murayama K, Wollnik B, Dattani M, Heath KE, Hickson ID, Jackson AP
American journal of human genetics 2018 Aug 2;103(2):221-231
American journal of human genetics 2018 Aug 2;103(2):221-231
BLM helicase regulates DNA repair by counteracting RAD51 loading at DNA double-strand break sites.
Patel DS, Misenko SM, Her J, Bunting SF
The Journal of cell biology 2017 Nov 6;216(11):3521-3534
The Journal of cell biology 2017 Nov 6;216(11):3521-3534
RECQ-like helicases Sgs1 and BLM regulate R-loop-associated genome instability.
Chang EY, Novoa CA, Aristizabal MJ, Coulombe Y, Segovia R, Chaturvedi R, Shen Y, Keong C, Tam AS, Jones SJM, Masson JY, Kobor MS, Stirling PC
The Journal of cell biology 2017 Dec 4;216(12):3991-4005
The Journal of cell biology 2017 Dec 4;216(12):3991-4005
FANCD2, FANCJ and BRCA2 cooperate to promote replication fork recovery independently of the Fanconi Anemia core complex.
Raghunandan M, Chaudhury I, Kelich SL, Hanenberg H, Sobeck A
Cell cycle (Georgetown, Tex.) 2015;14(3):342-53
Cell cycle (Georgetown, Tex.) 2015;14(3):342-53
TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation.
Blackford AN, Nieminuszczy J, Schwab RA, Galanty Y, Jackson SP, Niedzwiedz W
Molecular cell 2015 Mar 19;57(6):1133-1141
Molecular cell 2015 Mar 19;57(6):1133-1141
FANCD2-controlled chromatin access of the Fanconi-associated nuclease FAN1 is crucial for the recovery of stalled replication forks.
Chaudhury I, Stroik DR, Sobeck A
Molecular and cellular biology 2014 Nov;34(21):3939-54
Molecular and cellular biology 2014 Nov;34(21):3939-54
FANCD2 regulates BLM complex functions independently of FANCI to promote replication fork recovery.
Chaudhury I, Sareen A, Raghunandan M, Sobeck A
Nucleic acids research 2013 Jul;41(13):6444-59
Nucleic acids research 2013 Jul;41(13):6444-59
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The TOP3A antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human TOP3A. This antibody recognizes human, mouse, rat antigen. The TOP3A antibody has been validated for the following applications: ELISA, WB analysis.
