PA5-85901

antibody from Invitrogen Antibodies

Targeting: CEMIP2 TMEM2

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-85901

Provider

Invitrogen Antibodies

Product name

TMEM2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Endogenously-Produced Hyaluronan and Its Potential to Regulate the Development of Peritoneal Adhesions.

Kocurkova A, Nesporova K, Sandanusova M, Kerberova M, Lehka K, Velebny V, Kubala L, Ambrozova G
Biomolecules 2021 Dec 29;12(1)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 HA signal transduction is involved in PA development. Peritoneal membrane from mouse PA model 3 days after induction. In H&E images, fibrotic section was apparent (*). H&E-stained samples (DiaPath, Bergamo, Italy) were imaged by a light microscope (Nikon Eclipse 50i, Tokio, Japan) with a Plan Apo 4x/0.2 Nikon JAPAN objective. Images were processed using NIS elements software (Nikon, Tokio, Japan). Nuclei of cells are stained purple, extracellular matrix and cytoplasm are stained pink. Immunofluorescent staining revealed enhanced expression pattern for HA receptor CD44 (Abcam, #ab157107, 1/500, Cambridge, GB) in profibrotically activated peritoneal membrane, while expression of typical MCs marker podoplanin (PDPN, Abcam, #ab11936, 1/100, Cambridge, GB) and hyaluronidase TMEM2 (Invitrogen, #PA5-85901, 1/100, Waltham, Massachusetts, USA) in both quiescent and pro-fibrotically activated peritoneal membranes remained homogenous. Nuclei of cells were stained using ProLong(tm) Diamond Antifade Mountant with DAPI (Invitrogen, #P36962, Waltham, MA, USA). Peritoneal membrane was imaged using a laser scanning confocal microscope (Leica TCS SP8X, Wetzlar, Germany) with the Leica HC PL APO CS2 20x/0.75 IMM (Zoom 1) objective. Images were processed using Leica LasX software and are representative of three mice. Abbreviations: PA: peritoneal adhesions, H&E: Hematoxylin and Eosin, PDPN: podoplanin, DAPI: 40,6-diamidino-2-phenylindole.