sc-638

antibody from Santa Cruz Biotechnology

Targeting: PRKD1 PKC-mu, PKCM, PKD, PRKCM

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-638

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-638, RRID:AB_2253119

Product name

Anti-PRKD1

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

Submitted references

Regulation of Protein Kinase D During Differentiation and Proliferation of Primary Mouse Keratinocytes

M Ernest Dodd, Vladimir L Ristich, Sagarika Ray, Robert M Lober, Wendy B Bollag
Journal of Investigative Dermatology 2005 Aug;125(2):294-306

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 102

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human stomach shows distinct cytoplasmic and slightly weaker nuclear positivity in parietal cells of gastric glands.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.