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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS4862 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- PKD1 (phospho S910) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human PKD1 around the phosphorylation site of Serine 910.
- Description
- Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction. Protein kinase D (PKD), also called PKCÃ×, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway. In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCÃ×. Phosphorylation at Ser916 correlates with PKD/PKCÃ× catalytic activity.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-PKD1 (S910) pAb at 1:500 dilutionLane1:HEK293T cell lysate treated with PMA(100nM,15mins)Lane2:NIH-3T3 cell lysate treated with PMA(100nM,15mins)Lane3:H9C2 cell lysate treated with PMA(100nM,15mins)
