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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- AM32491SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti PRKD1 pSer916
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- KLH-conjugated, synthetic peptide containing ...RVpSIL.... in which pS corresponds to Phospho-Serine 916 of Mouse PKD
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- MC29
- Vial size
- 0.1 ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot Analysis (Representative image from a previous lot): Unstimulated (lanes 1 and 3) or PDBu-stimulated (lanes 2 and 4) HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-PKD Cat.-No AM32491SU-N (1/2,000, lanes 1 and 2) or Rabbit anti-PKD2 Polyclonal Antibody Cat.-No AP32448PU-N (1/5,000, lanes 3 and 4).Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.Arrow indicates phospho-PKD (110 kDa).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot Analysis (Representative lot data): Unstimulated (lane 2) or PDBu-stimulated (lane 1) HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-PKD.Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-PKD (110 kDa).
