GTX60928

antibody from GeneTex

Targeting: PRKD1 PKC-mu, PKCM, PKD, PRKCM

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX60928

Provider

GeneTex

Product name

PKC mu (phospho Ser742) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Peptide Competition and Phosphatase Treatment. Lysates prepared from K562 cells left unstimulated (1) or stimulated with PMA (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-5) or treated with lambda phosphatase (6), blocked with a 3% milk-TBST buffer for one hour at room temperature, and incubated with PKC?/PKD [pS742] antibody for two hours at room temperature in a 3% milk-TBST buffer, following prior incubation with: no peptide (1, 2, 6), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab"e;)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal? method. The data show that only the peptide corresponding to PKC?/PKD [pS742] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.