Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-C742544 - Provider product page
- Provider
- LSBio
- Product name
- PRKD1 / PKC Mu Antibody (aa233-246) LS-C742544
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1 was used to immunoprecipitate PKD1 with 2ug antibody. The precipitate was subsequently probed in Western blot using antibody at 1ug/ml. The secondary anti-goat picks up the heavy chain of antibody used
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1, Human DYKDDDDK-tagged PKD2 or Human GST-tagged PKD3 probed with Goat anti-PRKD1 (aa233-246) Antibody (0.1ug/ml) in top panel, probed with anti-DYKDDDDK in middle panel and probed with anti-GST in bottom panel. Data kindly obtained from Dr Peter Storz, Mayo Clinic, USA.