GTX17945

antibody from GeneTex

Targeting: PRKD1 PKC-mu, PKCM, PKD, PRKCM

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX17945

Provider

GeneTex

Proper citation

GeneTex Cat#GTX17945, RRID:AB_422988

Product name

PKC mu antibody

Antibody type

Polyclonal

Antigen

Synthetic phosphopeptide derived from a region of human PKC mu that contains serine 742. The sequence is conserved in mouse. (unfortunately, the amino acid sequence is considered to be commercially sensitive)

Host

Rabbit

Isotype

IgG

Vial size

50µl

Storage

Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Lysates prepared from K562 cells left unstimulated (1) or stimulated with PMA (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-5) or treated with lambda phosphatase (6), blocked with a 3% milk-TBST buffer for one hour at room temperature, and incubated with PKCµ/PKD [pS742] antibody for two hours at room temperature in a 3% milk-TBST buffer, following prior incubation with: no peptide (1, 2, 6), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to PKCµ/PKD [pS742] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific

Validation comment

WB