PA5-34810

antibody from Invitrogen Antibodies

Targeting: ERG erg-3, p55

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34810

Provider

Invitrogen Antibodies

Product name

ERG Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, H1299, HeLa, Molt-4, Raji, mouse brain. Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (85%), Xenopus laevis (96%), Pig (99%), Chicken (98%), Bovine (99%). IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ERG Polyclonal Antibody (Product # PA5-34810). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. ERG Polyclonal Antibody (Product # PA5-34810) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ERG Polyclonal Antibody (Product # PA5-34810). Sample (30 µg of whole cell lysate). Lane A: Molt-4. 10% SDS PAGE. ERG Polyclonal Antibody (Product # PA5-34810) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ERG Polyclonal Antibody (Product # PA5-34810). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. ERG Polyclonal Antibody (Product # PA5-34810) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-ERG Polyclonal Antibody (Product # PA5-34810) and a band of ~52 kDa corresponding to ERG was observed across the cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), A-431 (Lane 3), HEK-293 (Lane 4) and MOLT-4 (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).