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Learn14356-1-AP
antibody from Invitrogen Antibodies
Targeting: ERG
erg-3, p55
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [10]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 14356-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: RQWLEWAVK EYGLPDVNIL LFQNIDGKEL CKMTKDDFQR LTPSYNADIL LSHLHYLRET PLPHLTSDDV DKALQNSPRL MHARNTGGAA FIFPNTSVYP EATQRITTRP DLPYEPPRRS AWTGHGHPTP QSKAAQPSPS TVPKTEDQRP QLDPYQILGP TSSRLANPGS GQIQLWQFLL ELLSDSSNSS CITWEGTNGE FKMTDPDEVA RRWGERKSKP NMNYDKLSRA LRYYYDKNIM TKVHGKRYAY KFDFHGIAQA LQPHPPESSL YKYPSDLPYM GSYHAHPQKM NFVAPHPPAL PVTSSSFFAA PNPYWNSPTG GIYPNTRLPT SHMPSHLGTY Y (140-479 aa encoded by BC040168)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP ( ERG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA) fixed MCF-7 cells using 14356-1-AP (ERG antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 14356-1-AP (ERG antibody) at dilution of 1:25 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 14356-1-AP (ERG antibody) at dilution of 1:25 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 14356-1-AP (ERG antibody) at dilution of 1:25 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 14356-1-AP (ERG antibody) at dilution of 1:25 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 14356-1-AP (ERG antibody) at dilution of 1:25 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 14356-1-AP (ERG antibody) at dilution of 1:25 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 14356-1-AP (ERG antibody) at dilution of 1:25 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung using 14356-1-AP (ERG antibody) at dilution of 1:25 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung using 14356-1-AP (ERG antibody) at dilution of 1:25 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 14356-1-AP (ERG antibody) at dilution of 1:25 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 MCF-7 cells were intracellularly stained with 0.4 µg Anti-Human ERG (Product # 14356-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.4 µg Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ERG (IP:14356-1-AP, 4ug; Detection:14356-1-AP 1:500) with MCF-7 cells lysate 800ug.
