PA5-29594

antibody from Invitrogen Antibodies

Targeting: ERG erg-3, p55

Antibody data

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Validation data

ERG protein structure - PA5-29594 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ERG Polyclonal Antibody detects ERG protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract. B. 30 µg HepG2 whole cell lysate/extract.10 % SDS-PAGE. ERG Polyclonal Antibody (Product # PA5-29594) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ERG Polyclonal Antibody detects ERG protein by Western blot analysis. A. 30 µg Raw264.7 whole lysate/extract.10 % SDS-PAGE. ERG Polyclonal Antibody (Product # PA5-29594) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-ERG Polyclonal Antibody (Product # PA5-29594) and bands of ~50, 52 and 55 kDa corresponding to different isoforms of ERG was observed in Reh and HEL 92.1.7 cell lines and not in HT-29 and HCT 116 which are reported to be negative. Modified whole cell extracts (1% SDS) (30 µg lysate) of Reh (Lane 1), HEL 92.1.7 (Lane 2), HT- 29 (Lane 3) and HCT 116 (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).