Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-B2963 - Provider product page
- Provider
- LSBio
- Product name
- Anti-YWHAG / 14-3-3 Gamma Antibody (aa1-247, clone J3H10) IHC-plus™ LS-B2963
- Antibody type
- Monoclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- J3H10
- Vial size
- 50µg
- Storage
- Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Cell lysates of HeLa(30 ug) and mouse brain(30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 gamma (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-YWHAG / 14-3-3 Gamma antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2963 concentration 5 ug/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-YWHAG / 14-3-3 Gamma antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2963 concentration 5 ug/ml.