PA1-86556

antibody from Invitrogen Antibodies

Targeting: YWHAG 14-3-3GAMMA, 14-3-3γ, PPP1R170

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA1-86556

Provider

Invitrogen Antibodies

Product name

Anti-14-3-3 gamma Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Predicted to react with all mammals based on sequence homology. Heat-mediated antigen retrieval is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is normal brain tissue. In Western blot, this antibody detects a band at ~30kDa in HEK293 cell lysate.

Reactivity

Human, Mouse, Rat, Bovine, Chicken/Avian

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

4°C or -20°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of 14-3-3 isoforms in HEK293 cell extracts using a 14-3-3 gamma polyclonal antibody (Product # PA1-86556) (lane 1), a zeta 14-3-3 (lane 2), a C-epsilon-14-3-3 (lane 3), a N-epsilon-14-3-3 (Lane 4), a eta 14-3-3 (Lane 5). The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3.