Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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Validation data
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- Product number
- NBP2-50579 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal 14-3-3 gamma Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. Recombinant Human YWHAZ Lane 2. Recombinant Human YWHAB Lane 3. Recombinant Human YWHAE Lane 4. Recombinant Human YWHAH Lane 5.: Recombinant Human YWHAG Lane 6.: Recombinant Human SFN Lane 7.: Recombinant Human YWHAQ.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - The lysates of Hela (40ug) and Mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Lane 1.: 293T cell lysate Lane 2.: A431 cell lysate Lane 3.: K562 cell lysate Lane 4.: NIH3T3 cell lysate