F54406

antibody from NSJ Bioreagents

Targeting: YWHAG 14-3-3GAMMA, 14-3-3γ, PPP1R170

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54406

Provider

NSJ Bioreagents

Product name

YWHAG Antibody / 14-3-3 gamma

Antibody type

Polyclonal

Description

This highly specific YWHAG antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the YWHAG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse cerebellum lysate with YWHAG antibody. Predicted molecular weight ~28 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with YWHAG antibody.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human colon carcinoma tissue with YWHAG antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human WiDr cells with YWHAG antibody; Blue=isotype control, Green= YWHAG antibody.