Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA1-16588 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 14-3-3 gamma Monoclonal Antibody (HS23)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Suggested positive control: antigen standard for YWHAG (transient overexpression lysate), Hela whole cell extract.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Zebrafish
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HS23
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 14-3-3 gamma in Lane 1: HeLa cell lysate, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively). Samples were incubated in 14-3-3 gamma monoclonal antibody (Product # MA1-16588).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 14-3-3 gamma in Lane 1: HeLa cell lysate, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively). Samples were incubated in 14-3-3 gamma monoclonal antibody (Product # MA1-16588).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 14-3-3 gamma in HeLa (A), A431 (B), NIH3T3 (C) and PC-12 (D) cell lysate. Samples were incubated in 14-3-3 gamma monoclonal antibody (Product # MA1-16588) using a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of 14-3-3 gamma (green) in HepG2 cells using Product # MA1-16588. Nuclei (Blue) are counterstained with Hoechst 33258.