MA1-16587

antibody from Invitrogen Antibodies

Targeting: YWHAG 14-3-3GAMMA, 14-3-3γ, PPP1R170

Antibody data

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Validation data

Product number: MA1-16587 - Provider product page
Provider: Invitrogen Antibodies
Product name: 14-3-3 gamma Monoclonal Antibody (KC21)
Antibody type: Monoclonal
Antigen: Other
Description: Suggested positive control: antigen standard for YWHAG (transient overexpression lysate), HeLa whole cell extract.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: KC21
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

YWHAG protein structure - MA1-16587 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 14-3-3 gamma detected in lysates using Product # MA1-16587. Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of 14-3-3 gamma in Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively). Samples were incubated in 14-3-3 gamma monoclonal antibody (Product # MA1-16587).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of 14-3-3 gamma in 1.0 mg/mL HeLa lysate. Samples were incubated in 14-3-3 gamma monoclonal (Product # MA1-16587). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of 14-3-3 gamma in (1) HeLa, (2) PC12 and (3) NIH-3T3 whole cell lysates. Samples were incubated in 14-3-3 gamma monoclonal antibody (Product # MA1-16587) using a dilution of 2 µg/mL followed by an anti-rabbit HRP secondary antibody at a dilution of 1:10,000. The protein was detected at approximately 28 kDa after incubation with chemiluminescent substrate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of 14-3-3 gamma in Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively). Samples were incubated in 14-3-3 gamma monoclonal antibody (Product # MA1-16587).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of 14-3-3 gamma in 1.0 mg/mL HeLa lysate. Samples were incubated in 14-3-3 gamma monoclonal (Product # MA1-16587). This experiment was performed under reducing conditions using the 12-230 kDa separation system.