PA1-33115

antibody from Invitrogen Antibodies

Targeting: PPP1R15A GADD34

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-33115

Provider

Invitrogen Antibodies

Product name

GADD34 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX74479-PEP)..

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GADD34 in HEPG2 cell lysate (35 µg protein in RIPA buffer) using a GADD34 polyclonal antibody (Product # PA1-33115) at a dilution of 0.1 µg/mL following incubation for 1 hour and detected using chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GADD34 was performed by loading 35 µg (in RIPA buffer) of HepG2 (A) and negative control KLY (B) lysates. Proteins were transferred to a membrane and probed with a GADD34 Polyclonal Antibody (Product # PA1-33115) at a dilution of 0.3 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GADD34 was performed by loading 35 µg (in RIPA buffer) of HepG2 (A) and negative control KLY (B) lysates. Proteins were transferred to a membrane and probed with a GADD34 Polyclonal Antibody (Product # PA1-33115) at a dilution of 0.3 µg/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GADD34 in PFA fixed HeLa cells. Cells were permeabilized with 0.15% Triton and GADD34 Polyclonal Antibody (Product # PA1-33115) (Green) at a dilution of 10 µg/mL was used as a primary antibody. Blue: DAPI. Negative control : Unimmunized goat IgG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GADD34 in PFA fixed HepG2 cells. Cells were permeabilized with 0.15% Triton and GADD34 Polyclonal Antibody (Product # PA1-33115) (Green) at a dilution of 10 µg/mL was used as a primary antibody. Blue: DAPI. Negative control : Unimmunized goat IgG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of GADD34 in human liver tissue using GADD34 Polyclonal Antibody (Product # PA1-33115) at a dilution of 2 µg/mL. Antigen retrieval : citrate buffer pH 6.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of TLR7 was performed in HepG2 cells (fixed with PFA, permeabilized with 0.5% Triton) using a TLR7 Polyclonal Antibody (Product # PA1-28109) (Blue) at a dilution of 10 µg/mL. Black : Isotype control.