Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C55303 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C55303, RRID:AB_2168729
- Product name
- PPP1R15A / GADD34 Antibody (aa664-674) LS-C55303
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Antibody (0.1 ug/ml) staining of HEPG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- PPP1R15A / GADD34 antibody (0.3µg/ml) staining of HepG2 (A) and negative control KLY (B) lysate (35µg protein in RIPA buffer) Detected by chemiluminescence.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- PPP1R15A / GADD34 antibody (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.