Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- R33103 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GADD34 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific GADD34 antibody is suitable for use in Western blot/Immunohistochemistry/Immunocytochemistry/Immunofluorescence/Flow cytometry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the GADD34 antibody at -20oC.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HEPG2 cell lysate with GADD34 antibody at 0.1ug/ml. Predicted molecular weight ~73kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) HEPG2 cell lysate and 2) KLY negative control cell lysate with GADD34 antibody at 0.1ug/ml. Predicted molecular weight ~73 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF testing of fixed and permeabilized human HepG2 cells with GADD34 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF testing of fixed and permeabilized human HeLa cells with GADD34 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver with GADD34 antibody at 2ug/ml. HIER: steamed with pH6 citrate buffer, HRP-staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human HepG2 cells with GADD34 antibody (blue) at 10ug/10^6 cells and naive goat Ig (black).
