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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- R33103 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GADD34 Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids AAALDLSGRRG were used as the immunogen for this GADD34 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the GADD34 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HEPG2 cell lysate with GADD34 antibody at 0.1ug/ml. Predicted molecular weight ~73kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) HEPG2 cell lysate and 2) KLY negative control cell lysate with GADD34 antibody at 0.1ug/ml. Predicted molecular weight ~73 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF testing of fixed and permeabilized human HepG2 cells with GADD34 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF testing of fixed and permeabilized human HeLa cells with GADD34 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver with GADD34 antibody at 2ug/ml. HIER: steamed with pH6 citrate buffer, HRP-staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human HepG2 cells with GADD34 antibody (blue) at 10ug/10^6 cells and naive goat Ig (black).
