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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- RQ7100 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PPP1R15A Antibody / GADD34
- Antibody type
- Polyclonal
- Description
- This highly specific PPP1R15A antibody is suitable for use in Western blot/Immunohistochemistry/Direct ELISA applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PPP1R15A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human K562, 2) human HEL, 3) human RT4, 4) human U-251, 5) human SiHa, 6) human U-87 MG, 7) human PC-3 and 8) rat testis tissue lysate with PPP1R15A antibody. Predicted molecular weight ~73 kDa, can be observed at up to ~110 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human melanoma tissue with PPP1R15A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
