PA5-30486

antibody from Invitrogen Antibodies

Targeting: PPP1R15A GADD34

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30486

Provider

Invitrogen Antibodies

Product name

GADD34 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, Jurkat, Raji. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Upregulation of tRNA-Ser-AGA-2-1 Promotes Malignant Behavior in Normal Bronchial Cells.

Santos M, Fidalgo A, Varanda AS, Soares AR, Almeida GM, Martins D, Mendes N, Oliveira C, Santos MAS
Frontiers in molecular biosciences 2022;9:809985

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using GADD34 Polyclonal Antibody (Product # PA5-30486). Sample (30 µg of whole cell lysate). Lane A: 293T . 7.5% SDS PAGE. GADD34 Polyclonal Antibody (Product # PA5-30486) diluted at 1:1,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 2 UPR and UPS deregulation induced by recombinant tRNASer(AGA). (A) Expression of tRNA Ser (AGA) increases BiP expression in BEAS-2B cells. (B) Levels of eIF2 alpha -P were decreased in cells with tRNA Ser (AGA). (C) tRNASer(AGA) expression in BEAS-2B cell line increased the activation of ATF4 transcription factor. (D) Expression of GADD34 was increased in BEAS-2B cells expressing the recombinant tRNA Ser (AGA). (E) Immunoblots using antibodies against BiP, eIF2 alpha -P, eIF2 alpha , ATF4-P, ATF4 and GADD34. Beta-tub served as protein loading control. Graphics depict average +-SEM ( n = 3), with three technical replicates. (F) No differences are observed between BEAS-2B Mock and tRNA Ser (AGA) cells regarding ubiquitin levels. Graphic represents average +-SEM of five independent experiments. (G) Upregulation of tRNA Ser (AGA) in BEAS-2B increases proteasome activity by 1.32-fold. Graphic depicts average +-SEM ( n = 4), with three technical replicates. (H) Expression of ubiquitin. ss -tubulin represents the internal control. Total protein was extracted from BEAS-2B Mock cells (lanes 1, 2 and 3) and BEAS-2B tRNA Ser (AGA) (lanes 4, 5 and 6). Image represents results from one independent experiment with all technical replicates. Data was analyzed with One-way ANOVA and Holm Sidak's post-test and significant p values are shown (* p < 0.05; ** p < 0.01; *** p < 0.001).