LS-C174434

antibody from LSBio

Targeting: MAP2K3 MAPKK3, MEK3, MKK3, PRKMK3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C174434

Provider

LSBio

Product name

MAP2K3 / MEK3 / MKK3 Antibody (clone OTI2D7) LS-C174434

Antibody type

Monoclonal

Description

Protein A/G purified

Reactivity

Human, Mouse, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

OTI2D7

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-MAP2K3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K3.