Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-17216 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-MEK3 Monoclonal Antibody (3G2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- A suggested positive control for this product is HepG2 cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3G2
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MEK3 using anti-MEK3 monoclonal antibody (Product # MA5-17216) on (Lane 1) A431 Cell lysate, (Lane 2) HepG2 Cell lysate, (Lane 3) NIH3T3 Cell lysate and (Lane 4) C6 Cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MEK3 Rabbit Monoclonal Antibody (Product # MA5-17216) and 39 kDa, 36 kDa band corresponding to MEK3 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of Hela (Lane 1), A-431 (Lane 2), Hep G2 (Lane 3), C2C12 (Lane 4), U-87MG (Lane 5) and IMR-32 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).