MA5-17216

antibody from Invitrogen Antibodies

Targeting: MAP2K3 MAPKK3, MEK3, MKK3, PRKMK3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17216

Provider

Invitrogen Antibodies

Product name

Anti-MEK3 Monoclonal Antibody (3G2)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

A suggested positive control for this product is HepG2 cells.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

3G2

Vial size

100 µL

Concentration

2 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MEK3 using anti-MEK3 monoclonal antibody (Product # MA5-17216) on (Lane 1) A431 Cell lysate, (Lane 2) HepG2 Cell lysate, (Lane 3) NIH3T3 Cell lysate and (Lane 4) C6 Cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-MEK3 Rabbit Monoclonal Antibody (Product # MA5-17216) and 39 kDa, 36 kDa band corresponding to MEK3 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of Hela (Lane 1), A-431 (Lane 2), Hep G2 (Lane 3), C2C12 (Lane 4), U-87MG (Lane 5) and IMR-32 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).