H00005606-D01P

antibody from Abnova Corporation

Targeting: MAP2K3 MAPKK3, MEK3, MKK3, PRKMK3

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005606-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005606-D01P, RRID:AB_1577145

Product name

MAP2K3 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human MAP2K3 protein.

Antigen sequence

MESPASSQPASMPQSKGKSKRKKDLRISCMSKPPA
PNPTPPRNLDSRTFITIGDRNFEVEADDLVTISEL
GRGAYGVVEKVRHAQSGTIMAVKRIRATVNSQEQK
RLLMDLDINMRTVDCFYTVTFYGALFREGDVWICM
ELMDTSLDKFYRKVLDKNMTIPEDILGEIAVSIVR
ALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFG
ISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGY
NVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQ
VVEEPSPQLPADRFSPEFVDFTAQCLRKNPAERMS
YLELMEHPFFTLHKTKKTDIAAFVKEILGEDS


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MAP2K3 expression in transfected 293T cell line (H00005606-T01) by MAP2K3 MaxPab polyclonal antibody.Lane 1: MAP2K3 transfected lysate(39.30 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between MAP2K3 and MAP3K4. HeLa cells were stained with anti-MAP2K3 rabbit purified polyclonal 1:1200 and anti-MAP3K4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)