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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NB100-82048 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-82048, RRID:AB_1144551
- Product name
- Rabbit Polyclonal MKK3/MEK3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. MKK3 (specific to Phospho-Ser189) detects endogenous levels of MKK3 only when phosphorylated at serine189.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references MKK3 regulates mitochondrial biogenesis and mitophagy in sepsis-induced lung injury.
Mannam P, Shinn AS, Srivastava A, Neamu RF, Walker WE, Bohanon M, Merkel J, Kang MJ, Dela Cruz CS, Ahasic AM, Pisani MA, Trentalange M, West AP, Shadel GS, Elias JA, Lee PJ
American journal of physiology. Lung cellular and molecular physiology 2014 Apr 1;306(7):L604-19
American journal of physiology. Lung cellular and molecular physiology 2014 Apr 1;306(7):L604-19
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Analysis of extract from MDA-MB-435 cells untreated or treated with UV, using MKK3 (Ab-189) antibody (Lane 1 and 2) and MKK3 (phospho-Ser189) antibody (Lane 3 and 4).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Analysis of extracts from Hela cells untreated or treated with PMA using MKK3(Phospho-Ser189) antibody
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using MKK3 (Phospho-Ser189) Antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using MKK3 (phospho-Ser189) antibody.
