MA5-32480

antibody from Invitrogen Antibodies

Targeting: DUSP1 CL100, HVH1, MKP-1, PTPN10

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32480

Provider

Invitrogen Antibodies

Product name

MKP-1 Recombinant Rabbit Monoclonal Antibody (JJ0930)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

JJ0930

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Knockdown of TRIM9 attenuates irinotecan‑induced intestinal mucositis in IEC‑6 cells by regulating DUSP6 expression via the P38 pathway.

Zhao W, Wang Q
Molecular medicine reports 2021 Dec;24(6)

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of MKP-1 in HepG2 cells using a MKP-1 Monoclonal Antibody (Product # MA5-32480) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. TRIM9 directly interacts with DUSP6 in IEC-6 cells. (A) The protein expression levels of TRIM9, DUSP1, DUSP4, DUSP5, DUSP6 and DUSP10 in IEC-6 cells transfected with oeTRIM9 was detected. **P