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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- PA5-62629 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZG16 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RSSSYSGEYG SGGGKRFSHS GNQLDGPITA LRVRVNTYYI VGLQVRYGKV WSDYVGGRNG DLEEIFLHPG E
- Concentration
- 0.05 mg/mL
Submitted references BMP gradient along the intestinal villus axis controls zonated enterocyte and goblet cell states.
Beumer J, Puschhof J, Yengej FY, Zhao L, Martinez-Silgado A, Blotenburg M, Begthel H, Boot C, van Oudenaarden A, Chen YG, Clevers H
Cell reports 2022 Mar 1;38(9):110438
Cell reports 2022 Mar 1;38(9):110438
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZG16 in control (vector only transfected HEK293T lysate) and ZG16 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a ZG16 Polyclonal Antibody (Product # PA5-62629).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZG16 in human colon using ZG16 Polyclonal Antibody (Product # PA5-62629).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZG16 in human testis using ZG16 Polyclonal Antibody (Product # PA5-62629).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZG16 in human rectum and liver tissues using ZG16 Polyclonal Antibody (Product # PA5-62629). Corresponding ZG16 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZG16 in human colon, liver, rectum and testis using ZG16 Polyclonal Antibody (Product # PA5-62629) (A) shows similar protein distribution across tissues to an independent ZG16 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZG16 in human liver using ZG16 Polyclonal Antibody (Product # PA5-62629) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZG16 in human rectum using ZG16 Polyclonal Antibody (Product # PA5-62629) shows high expression.
