HPA052066

antibody from Atlas Antibodies

Targeting: ZG16 hZG16, JCLN, JCLN1, ZG16A

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA052066

Provider

Atlas Antibodies

Proper citation

Product name

Anti-ZG16

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human ZG16, Gene description: zymogen granule protein 16, Alternative Gene Names: hZG16, JCLN, JCLN1, ZG16A, Validated applications: IHC, WB, Uniprot ID: O60844, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.05 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

A Novel Prognostic Score Based on ZG16 for Predicting CRC Survival

Wang W, Sun J, Wang X, Ying H, You X, Sun F
Pharmacogenomics and Personalized Medicine 2020;Volume 13

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and ZG16 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407589).

Sample type

Human

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human rectum and testis tissues using HPA052066 antibody. Corresponding ZG16 RNA-seq data are presented for the same tissues.

Sample type

Human

Protocol

Protocol

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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