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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF2848 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse ERK5/BMK1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse ERK5/BMK1 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q13164- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Memantine protects against ischemia/reperfusion-induced brain endothelial permeability.
Erk5 is a mediator to TGFβ1-induced loss of phenotype and function in human podocytes.
TGF-beta activates ERK5 in human renal epithelial cells.
Liu Y, Huang Y, Xu Y, Qu P, Wang M
IUBMB life 2018 Apr;70(4):336-343
IUBMB life 2018 Apr;70(4):336-343
Erk5 is a mediator to TGFβ1-induced loss of phenotype and function in human podocytes.
Badshah II, Baines DL, Dockrell ME
Frontiers in pharmacology 2014;5:71
Frontiers in pharmacology 2014;5:71
TGF-beta activates ERK5 in human renal epithelial cells.
Browne JA, Pearson AL, Zahr RA, Niculescu-Duvaz I, Baines DL, Dockrell ME
Biochemical and biophysical research communications 2008 Aug 29;373(3):440-4
Biochemical and biophysical research communications 2008 Aug 29;373(3):440-4
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse ERK5/BMK1 by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ERK5/BMK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2848) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ERK5/BMK1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- ERK5/BMK1 in HeLa Human Cell Line. ERK5/BMK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse ERK5/BMK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2848) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
