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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21666 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HepG2.
- Concentration
- 0.35 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NCS1 Polyclonal Antibody (Product # PA5-21666). Sample (30 µg of whole cell lysate). A: A431. B: Hep G2. 12% SDS PAGE. NCS1 Polyclonal Antibody (Product # PA5-21666) diluted at 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NCS-1 in methanol-fixed A431 cells using a NCS-1 polyclonal antibody (Product # PA5-21666) (Green) at a 1:100 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using NCS1 (Product # PA5-21666) antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NCS1 Polyclonal Antibody detects NCS1 protein at on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NCS1 Polyclonal Antibody (Product # PA5-21666) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
