H00005518-M02

antibody from Abnova Corporation

Targeting: PPP2R1A PP2A-Aalpha, PP2AA, PR65A

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005518-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005518-M02, RRID:AB_1711640

Product name

PPP2R1A monoclonal antibody (M02), clone 4E6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant PPP2R1A.

Antigen sequence

MAAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKL
STIALALGVERTRSELLPFLTDTIYDEDEVLLALA
EQLGTFATLVGGPEYVHCLLPPLESLATVEETVVR
DKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDW
FTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCS
DDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFS
NLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVM
PTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITK
TDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSA
DCRENVIMSQILPCIKELVSDANQHVKSALASVIM
GLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNII
SNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVR
LAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHV
YAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMS
GDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTV
LRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEV
KPILEKLTQDQDVDVKYFAQEALTVLSLA


Isotype

IgG

Antibody clone number

4E6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PPP2R1A monoclonal antibody (M02), clone 4E6. Western Blot analysis of PPP2R1A expression in Jurkat(Cat # L017V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PPP2R1A is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between TP53 and PPP2R1A. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PPP2R1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)