PA5-77089
antibody from Invitrogen Antibodies
Targeting: SSX2
CT5.2a, HD21, HOM-MEL-40, MGC119055, MGC15364, MGC3884, SSX
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-77089 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SSX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Novel TCR-like CAR-T cells targeting an HLA∗0201-restricted SSX2 epitope display strong activity against acute myeloid leukemia.
Raskin S, Van Pelt S, Toner K, Balakrishnan PB, Dave H, Bollard CM, Yvon E
Molecular therapy. Methods & clinical development 2021 Dec 10;23:296-306
Molecular therapy. Methods & clinical development 2021 Dec 10;23:296-306
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of SSX2 in HeLa cells. The samples were probed with a SSX2 polyclonal antibody (Product # PA5-77117) followed by DAPI to stain cell nuclei.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 SSX2 expression on the AML cell lines THP-1 and KG1a (A) Expression of SSX2 by the KG1a and THP1 AML cell lines was detected by flow cytometry analysis. The SR cell line was used as a negative control for SSX2 expression. (B) Phenotypic analysis of the THP-1 cell line shows HLA-A2 expression, as well as expression of CD33 and the costimulatory molecules CD80 and CD86. The KG1a cell line displays a similar phenotypic profile with THP1 but lacks expression of HLA-A2.