Antibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 11-0419-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD41a Monoclonal Antibody (HIP8), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HIP8 monoclonal antibody reacts with the human CD41 molecule, the integrin alphaIIb also known as platelet GPIIb. CD41 non-covalently associates with integrin beta3 (GPIIIa, CD61) and is expressed by megakaryocytes and platelets. The CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin and mediates platelets aggregation. HIP8 blocks platelet aggregation. Applications Reported: The HIP8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIP8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- HIP8
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia.
In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool.
Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.
Resolving the immune landscape of human prostate at a single-cell level in health and cancer.
Platelets Fuel the Inflammasome Activation of Innate Immune Cells.
Yersinia pestis escapes entrapment in thrombi by targeting platelet function.
Platelet-Derived GARP Induces Peripheral Regulatory T Cells-Potential Impact on T Cell Suppression in Patients with Melanoma-Associated Thrombocytosis.
SARS-CoV-2 Receptors are Expressed on Human Platelets and the Effect of Aspirin on Clinical Outcomes in COVID-19 Patients.
Pollen-derived RNAs Are Found in the Human Circulation.
T cell-derived soluble glycoprotein GPIbα mediates PGE(2) production in human monocytes activated with the vaccine adjuvant MDP.
Mitochondrial DNA in the tumour microenvironment activates neutrophils and is associated with worse outcomes in patients with advanced epithelial ovarian cancer.
The role of platelets in mediating a response to human influenza infection.
PD-L1 is expressed on human platelets and is affected by immune checkpoint therapy.
The Plant Hormone Abscisic Acid Is a Prosurvival Factor in Human and Murine Megakaryocytes.
Association between toll-like receptor 6 expression and auxiliary T cells in the peripheral blood of pediatric patients with allergic purpura.
Bone marrow stromal cells transduced with a thrombopoietin, interleukin-6, and interleukin-11 syncretic gene induce cord mononuclear cells to generate platelets in vitro.
Circulating microparticles carry oxidation-specific epitopes and are recognized by natural IgM antibodies.
Constitutively released adenosine diphosphate regulates proplatelet formation by human megakaryocytes.
Structural and therapeutic insights from the species specificity and in vivo antithrombotic activity of a novel alphaIIb-specific alphaIIbbeta3 antagonist.
Voit RA, Tao L, Yu F, Cato LD, Cohen B, Fleming TJ, Antoszewski M, Liao X, Fiorini C, Nandakumar SK, Wahlster L, Teichert K, Regev A, Sankaran VG
Nature immunology 2023 Jan;24(1):69-83
Nature immunology 2023 Jan;24(1):69-83
In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool.
Butov KR, Osipova EY, Mikhalkin NB, Trubina NM, Panteleev MA, Machlus KR
Platelets 2021 Oct 3;32(7):928-935
Platelets 2021 Oct 3;32(7):928-935
Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.
Louka E, Povinelli B, Rodriguez-Meira A, Buck G, Wen WX, Wang G, Sousos N, Ashley N, Hamblin A, Booth CAG, Roy A, Elliott N, Iskander D, de la Fuente J, Fordham N, O'Byrne S, Inglott S, Norfo R, Salio M, Thongjuea S, Rao A, Roberts I, Mead AJ
The Journal of experimental medicine 2021 Feb 1;218(2)
The Journal of experimental medicine 2021 Feb 1;218(2)
Resolving the immune landscape of human prostate at a single-cell level in health and cancer.
Tuong ZK, Loudon KW, Berry B, Richoz N, Jones J, Tan X, Nguyen Q, George A, Hori S, Field S, Lynch AG, Kania K, Coupland P, Babbage A, Grenfell R, Barrett T, Warren AY, Gnanapragasam V, Massie C, Clatworthy MR
Cell reports 2021 Dec 21;37(12):110132
Cell reports 2021 Dec 21;37(12):110132
Platelets Fuel the Inflammasome Activation of Innate Immune Cells.
Rolfes V, Ribeiro LS, Hawwari I, Böttcher L, Rosero N, Maasewerd S, Santos MLS, Próchnicki T, Silva CMS, Wanderley CWS, Rothe M, Schmidt SV, Stunden HJ, Bertheloot D, Rivas MN, Fontes CJ, Carvalho LH, Cunha FQ, Latz E, Arditi M, Franklin BS
Cell reports 2020 May 12;31(6):107615
Cell reports 2020 May 12;31(6):107615
Yersinia pestis escapes entrapment in thrombi by targeting platelet function.
Palace SG, Vitseva O, Proulx MK, Freedman JE, Goguen JD, Koupenova M
Journal of thrombosis and haemostasis : JTH 2020 Dec;18(12):3236-3248
Journal of thrombosis and haemostasis : JTH 2020 Dec;18(12):3236-3248
Platelet-Derived GARP Induces Peripheral Regulatory T Cells-Potential Impact on T Cell Suppression in Patients with Melanoma-Associated Thrombocytosis.
Zimmer N, Krebs FK, Zimmer S, Mitzel-Rink H, Kumm EJ, Jurk K, Grabbe S, Loquai C, Tuettenberg A
Cancers 2020 Dec 5;12(12)
Cancers 2020 Dec 5;12(12)
SARS-CoV-2 Receptors are Expressed on Human Platelets and the Effect of Aspirin on Clinical Outcomes in COVID-19 Patients.
Sahai A, Bhandari R, Koupenova M, Freedman JE, Godwin M, McIntyre T, Chung MK, Iskandar JP, Kamran H, Hariri E, Aggarwal A, Kalra A, Bartholomew JR, McCrae KR, Elbadawi A, Svensson LG, Kapadia S, Cameron SJ
Research square 2020 Dec 23;
Research square 2020 Dec 23;
Pollen-derived RNAs Are Found in the Human Circulation.
Koupenova M, Mick E, Corkrey HA, Singh A, Tanriverdi SE, Vitseva O, Levy D, Keeler AM, Ezzaty Mirhashemi M, ElMallah MK, Gerstein M, Rozowsky J, Tanriverdi K, Freedman JE
iScience 2019 Sep 27;19:916-926
iScience 2019 Sep 27;19:916-926
T cell-derived soluble glycoprotein GPIbα mediates PGE(2) production in human monocytes activated with the vaccine adjuvant MDP.
Liu F, Endo Y, Romantseva T, Wu WW, Akue A, Shen RF, Golding H, Zaitseva M
Science signaling 2019 Oct 8;12(602)
Science signaling 2019 Oct 8;12(602)
Mitochondrial DNA in the tumour microenvironment activates neutrophils and is associated with worse outcomes in patients with advanced epithelial ovarian cancer.
Singel KL, Grzankowski KS, Khan ANMNH, Grimm MJ, D'Auria AC, Morrell K, Eng KH, Hylander B, Mayor PC, Emmons TR, Lénárt N, Fekete R, Környei Z, Muthukrishnan U, Gilthorpe JD, Urban CF, Itagaki K, Hauser CJ, Leifer C, Moysich KB, Odunsi K, Dénes Á, Segal BH
British journal of cancer 2019 Jan;120(2):207-217
British journal of cancer 2019 Jan;120(2):207-217
The role of platelets in mediating a response to human influenza infection.
Koupenova M, Corkrey HA, Vitseva O, Manni G, Pang CJ, Clancy L, Yao C, Rade J, Levy D, Wang JP, Finberg RW, Kurt-Jones EA, Freedman JE
Nature communications 2019 Apr 16;10(1):1780
Nature communications 2019 Apr 16;10(1):1780
PD-L1 is expressed on human platelets and is affected by immune checkpoint therapy.
Rolfes V, Idel C, Pries R, Plötze-Martin K, Habermann J, Gemoll T, Bohnet S, Latz E, Ribbat-Idel J, Franklin BS, Wollenberg B
Oncotarget 2018 Jun 8;9(44):27460-27470
Oncotarget 2018 Jun 8;9(44):27460-27470
The Plant Hormone Abscisic Acid Is a Prosurvival Factor in Human and Murine Megakaryocytes.
Malara A, Fresia C, Di Buduo CA, Soprano PM, Moccia F, Balduini C, Zocchi E, De Flora A, Balduini A
The Journal of biological chemistry 2017 Feb 24;292(8):3239-3251
The Journal of biological chemistry 2017 Feb 24;292(8):3239-3251
Association between toll-like receptor 6 expression and auxiliary T cells in the peripheral blood of pediatric patients with allergic purpura.
Chang H, Cao Y, Lin YI, Zhu H, Fu Y, Chen X, Zhang Q
Experimental and therapeutic medicine 2015 Oct;10(4):1536-1540
Experimental and therapeutic medicine 2015 Oct;10(4):1536-1540
Bone marrow stromal cells transduced with a thrombopoietin, interleukin-6, and interleukin-11 syncretic gene induce cord mononuclear cells to generate platelets in vitro.
Lu H, Jiang T, Li R, Wang S, Zhang Q, Zhao S
Transfusion 2015 Jan;55(1):176-86
Transfusion 2015 Jan;55(1):176-86
Circulating microparticles carry oxidation-specific epitopes and are recognized by natural IgM antibodies.
Tsiantoulas D, Perkmann T, Afonyushkin T, Mangold A, Prohaska TA, Papac-Milicevic N, Millischer V, Bartel C, Hörkkö S, Boulanger CM, Tsimikas S, Fischer MB, Witztum JL, Lang IM, Binder CJ
Journal of lipid research 2015 Feb;56(2):440-8
Journal of lipid research 2015 Feb;56(2):440-8
Constitutively released adenosine diphosphate regulates proplatelet formation by human megakaryocytes.
Balduini A, Di Buduo CA, Malara A, Lecchi A, Rebuzzini P, Currao M, Pallotta I, Jakubowski JA, Cattaneo M
Haematologica 2012 Nov;97(11):1657-65
Haematologica 2012 Nov;97(11):1657-65
Structural and therapeutic insights from the species specificity and in vivo antithrombotic activity of a novel alphaIIb-specific alphaIIbbeta3 antagonist.
Blue R, Kowalska MA, Hirsch J, Murcia M, Janczak CA, Harrington A, Jirouskova M, Li J, Fuentes R, Thornton MA, Filizola M, Poncz M, Coller BS
Blood 2009 Jul 2;114(1):195-201
Blood 2009 Jul 2;114(1):195-201
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of human platelets with Anti-Human CD42b APC and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD41a FITC (right). Total events were used for analysis.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure A2 Platelets bound to T cells upon activation in coculture. CD4 + CD25 - T cells were cocultured with or without platelets at the ratio of 1:50 and stimulated with 0.5 ug/mL anti-CD3 mAb and 1.0 ug/mL anti-CD28 mAb for 6 days. At day 1 and 6, platelet-T cell conjugates (CD41a + CD4 + double-positive cells) were analyzed via flow cytometry. For assessment of GARP expression, only CD4 + CD41a- cells were included in the analysis (indicated by the pregating on the upper left (UL)). GARP expression was normalized to the untreated (w/o) control. Dot plots show one representative result of five independent experiments. Isotype controls are shown ( n = 5, box and whiskers, medians + min/max, ** p
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Ovarian cancer ascites induces rapid platelet activation and aggregation that is partially abrogated by DNase and protease treatments. a - g Ascites were collected from patients with newly diagnosed advanced EOC and 500 g supernatants (ASC) were used. a Floating aggregates were collected from ascites before centrifugation and analysed by H&E. Abundant neutrophils (black box) and a sparse number of tumour cells (black arrow) embedded in fibrin deposits (pink filaments) were identified. b , c CD41 + PMP were measured in ascites supernatants by modified flow cytometry ( n = 2). Variability exists between different patients (B, left) 16.58% and (C, left) 1.16% CD41a + . In parallel, samples were 0.1 um-filtered to remove microparticles as a specificity control ( b , c , right panels). d - g . Platelets were isolated from peripheral blood of healthy donors and murine cardiac puncture. d Donor platelets were exposed to Tyrode's buffer with 1 mM CaCl 2 (negative control) or ascites supernatants ( n = 7) in the presence of 1 mM CaCl 2 for 30 minutes prior to staining for flow cytometry. Representative density plots show increased number of P-selectin + platelets (P11 gate) after exposure to ascites supernatants, which is quantified to the right (**, p < 0.01 ). E) Naive murine platelets were exposed to the same ascites supernatants ( n = 7), resulting in increased P-selectin expression and loss of CD42d from the surface of platelets within 15 minutes. The proportion of platele
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Characterization of human blood from influenza-infected patients. a - d Blood from influenza-infected patients was fixed after intravenous collection and stained as described in Methods. In all cases DNA was assessed by DAPI. a Many platelets appeared similar to those observed in control blood from healthy donors with a size of 2-5 um. b Some platelets from influenza-infected patients had undergone spreading with a distinctive distribution of CD41. c Platelets and platelet microparticles associated with DNA (arrow). d Spread platelets were found surrounding released DNA with distinct DNA content in their center. Of note, blood from influenza-infected patients and controls in ( a - d ) was not permeabilized. Since formaldehyde can cause certain levels of permeabilization as a function of cross-linking positive staining in control samples for H4 and MPO do not necessarily indicate activation. e - g Levels of proteins related to DNA release in the plasma of influenza-infected patients assessed by ELISA. Source data are provided as a Source Data file. e neutrophil elastase, f myeloperoxidase (MPO), and g histone nucleosome core. The graphs represent the average +- SD of healthy donors ( n = 15) and influenza-infected patients ( n = 18); significance for ( e - g ) was assessed by Mann-Whitney U test, star symbol (*) indicates p < 0.0001. h , i To synchronize time of influenza presence as a function of infection and quantify the released DNA, we
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Expression of ACE2 and TMPRSS2 in Platelets by Confocal Microscopy. Platelets isolated from venous blood of healthy individuals was stained for 1h with the following antibodies: CD41 (platelet-specific marker), ACE2, TMPRSS2, and DAPI to eliminate any DNA components. Mounted slides were resolved by confocal fluorescent microscopy using a 100x objective lens. Images are representative of n=6 donors for ACE2 and n=3 for TMPRSS2. Each image represents a different donor. The scale bar is noted.
- Conjugate
- Green dye