PA5-33143

antibody from Invitrogen Antibodies

Targeting: ENO1 ENO1L1, MBP-1, MPB1, PPH

ELISA

Immunohistochemistry

Antibody data

Submit

Validation data

Product number: PA5-33143 - Provider product page
Provider: Invitrogen Antibodies
Product name: ENO1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: Rabbit anti Human non-neuronal enolase polyclonal antibody recognizes human non-neuronal enolase, also known as alpha enolase or enolase 1.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 1 mL
Concentration: 5 mg/mL
Storage: 4°C or -20°C if preferred

ENO1 protein structure - PA5-33143 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Alpha-enolase (ENO1) was achieved by transfecting U-87 MG cells with ENO1 specific siRNAs (Silencer® select Product # s4680). Western blot analysis (Fig. a) was performed using whole cell extracts from the ENO1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed using ENO1 Polyclonal Antibody (Product # PA5-33143, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ENO1. An uncharacterized band (*) t ~55 kDa was also seen across samples tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using anti-ENO1 Polyclonal Antibody (Product # PA5-33143) on whole cell extracts (30 µg lysate) of THP-1 (Lane1), A549 (Lane 2) and U-87 MG (Lane 3) and 47 kDa band corresponding to ENO1 protein was observed along with an uncharacterized band (*) at 55kDa. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25 µg/mL, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).