ABIN2508021

antibody from antibodies-online

Targeting: ENO1 ENO1L1, MBP-1, MPB1, PPH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN2508021

Provider

antibodies-online

Product name

anti-Enolase 1, (Alpha) (ENO1) antibody

Antibody type

Monoclonal

Antigen

Purified His-tagged ENO1 protein was used to produced this monoclonal antibody.Antigen type: Recombinant Protein

Description

This antibody is purified through a protein G column, followed by dialysis against PBS.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

200 μL

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: 1-2. Anti-GDNF Family Receptor ?4 (extracellular) antibody (ABIN2511162), (1:800). 3-4. Anti-GDNF Family Receptor ?4 (extracellular) antibody, preincubated with the control peptide antigen. Expression of GFR?4 in rat red nucleus Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GDNF Family Receptor ?4 (extracellular) antibody (ABIN2511162), (1:100). Staining reveals expression of GFR?4 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus. DAPI is used as the counterstain (blue). Expression of GFR?4 in rat PC12 cells Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. Extracellular staining of cells with Anti-GDNF Family Receptor ?4 (extracellular) antibody (ABIN2511162), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei stained with the cell permeable dye Hoechst 33342 (blue). C. Merge of the two images.

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Image(s): Immunofluorescence

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Image(s): Immunofluorescence