Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-59457-100ul - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Enolase 1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - Lane 1: PC3 cell lysates, Lane 2: MCF7 cell lysates, Lane 3: 293T cell lysates, Lane 4: HeLa cell lysates
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ENO1 (Alpha-enolase) recombinant protein Lane 2.: ENO2 (Gamma-enolase) recombinant protein Lane 3.: ENO3 (Beta-enolase) recombinant protein
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - The recombinant Enolase 1 protein [NBP1-30262] (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human [NBP2-59457] (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ENO1 (Alpha-enolase) recombinant protein.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis of ENO1, 2 in LNCap cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).